Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species.
Journal Article (Journal Article)
The known primate lentiviruses can be divided into two subgroups consisting of the human immunodeficiency virus type 1 (HIV-1) isolates and the related HIV type 2 (HIV-2) and simian immunodeficiency virus (SIV) isolates. HIV-1 has been shown to encode a post-transcriptional trans-activator of viral structural gene expression, termed Rev, that is essential for viral replication in culture. Here, we demonstrate that HIV-2 and SIVmac also encode functional Rev proteins. As in the case of HIV-1, these Rev trans-activators are shown to induce the cytoplasmic expression of the unspliced viral transcripts that encode the viral structural proteins. Unexpectedly, the Rev proteins of HIV-2 and SIVmac proved incapable of activating the cytoplasmic expression of unspliced HIV-1 transcripts, whereas HIV-1 Rev was fully functional in the HIV-2/SIV system. This nonreciprocal complementation may imply a direct role for Rev in mediating the recognition of its viral RNA target sequence.
Full Text
Duke Authors
Cited Authors
- Malim, MH; Böhnlein, S; Fenrick, R; Le, SY; Maizel, JV; Cullen, BR
Published Date
- November 1989
Published In
Volume / Issue
- 86 / 21
Start / End Page
- 8222 - 8226
PubMed ID
- 2682638
Pubmed Central ID
- PMC298252
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.86.21.8222
Language
- eng
Conference Location
- United States