Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species.

Published

Journal Article

The known primate lentiviruses can be divided into two subgroups consisting of the human immunodeficiency virus type 1 (HIV-1) isolates and the related HIV type 2 (HIV-2) and simian immunodeficiency virus (SIV) isolates. HIV-1 has been shown to encode a post-transcriptional trans-activator of viral structural gene expression, termed Rev, that is essential for viral replication in culture. Here, we demonstrate that HIV-2 and SIVmac also encode functional Rev proteins. As in the case of HIV-1, these Rev trans-activators are shown to induce the cytoplasmic expression of the unspliced viral transcripts that encode the viral structural proteins. Unexpectedly, the Rev proteins of HIV-2 and SIVmac proved incapable of activating the cytoplasmic expression of unspliced HIV-1 transcripts, whereas HIV-1 Rev was fully functional in the HIV-2/SIV system. This nonreciprocal complementation may imply a direct role for Rev in mediating the recognition of its viral RNA target sequence.

Full Text

Duke Authors

Cited Authors

  • Malim, MH; Böhnlein, S; Fenrick, R; Le, SY; Maizel, JV; Cullen, BR

Published Date

  • November 1989

Published In

Volume / Issue

  • 86 / 21

Start / End Page

  • 8222 - 8226

PubMed ID

  • 2682638

Pubmed Central ID

  • 2682638

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.21.8222

Language

  • eng

Conference Location

  • United States