Cloning and expression of the yeast plasma membrane ATPase in Escherichia coli.

Published

Journal Article

The yeast plasma membrane ATPase gene PMA1 was cloned into Escherichia coli using the high expression tac and T7 promoters. The gene product is toxic to the bacterial cell leading to very low expression levels and arrested growth of the host cell within minutes of induction. The expressed protein is immunologically cross-reactive with the yeast ATPase, comigrates with the original protein in sodium dodecyl sulfate-polyacrylamide gels, and is isolated in the E. coli membrane fraction. The partially purified protein exhibits ATPase activity.

Full Text

Duke Authors

Cited Authors

  • Holzer, KP; Hammes, GG

Published Date

  • August 25, 1989

Published In

Volume / Issue

  • 264 / 24

Start / End Page

  • 14389 - 14395

PubMed ID

  • 2527233

Pubmed Central ID

  • 2527233

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States