Interaction of dihydrofolate reductase with methotrexate: ensemble and single-molecule kinetics.

Journal Article (Journal Article)

The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N terminus of the molecule. The fluorescent molecule was placed on a protein loop that closes over methotrexate when binding occurs, thus causing a quenching of the fluorescence. The biotin was used to attach the enzyme in an active form to a glass surface for single-molecule studies. The equilibrium dissociation constant for the binding of methotrexate to the enzyme is 9.5 nM. The stopped-flow studies revealed that methotrexate binds to two different conformations of the enzyme, and the association and dissociation rate constants were determined. The single-molecule investigation revealed a conformational change in the enzyme-methotrexate complex that was not observed in the stopped-flow studies. The ensemble averaged rate constants for this conformation change in both directions is about 2-4 s(-1) and is attributed to the opening and closing of the enzyme loop over the bound methotrexate. Thus the mechanism of methotrexate binding to DHFR involves multiple steps and protein conformational changes.

Full Text

Duke Authors

Cited Authors

  • Rajagopalan, PTR; Zhang, Z; McCourt, L; Dwyer, M; Benkovic, SJ; Hammes, GG

Published Date

  • October 15, 2002

Published In

Volume / Issue

  • 99 / 21

Start / End Page

  • 13481 - 13486

PubMed ID

  • 12359872

Pubmed Central ID

  • PMC129699

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.172501499


  • eng

Conference Location

  • United States