A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase.
Publication
, Journal Article
Wolfman, NM; Hammes, GG
Published in: J Biol Chem
December 25, 1979
The heat of interaction of ATP with phosphofructokinase from rabbit muscle was determined at 25 degrees C in 0.1 M potassium phosphate, pH 7.0 and 8.0. The limiting value of the enthalpy change at high ATP concentrations was found to be -11.5 kcal mol of enzyme polypeptide chains. Since phosphate and imidazole have very different heats of ionization (+0.8 and +7.5 kcal/mol, respectively), this suggests that the binding of at least two protons to the enzyme occurs concomitantly with the binding of ATP at the regulatory site.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
December 25, 1979
Volume
254
Issue
24
Start / End Page
12289 / 12290
Location
United States
Related Subject Headings
- Rabbits
- Phosphofructokinase-1
- Muscles
- Hydrogen-Ion Concentration
- Calorimetry
- Biochemistry & Molecular Biology
- Animals
- Adenosine Triphosphate
- 11 Medical and Health Sciences
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Wolfman, N. M., & Hammes, G. G. (1979). A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase. J Biol Chem, 254(24), 12289–12290.
Wolfman, N. M., and G. G. Hammes. “A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase.” J Biol Chem 254, no. 24 (December 25, 1979): 12289–90.
Wolfman NM, Hammes GG. A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase. J Biol Chem. 1979 Dec 25;254(24):12289–90.
Wolfman, N. M., and G. G. Hammes. “A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase.” J Biol Chem, vol. 254, no. 24, Dec. 1979, pp. 12289–90.
Wolfman NM, Hammes GG. A calorimetric study of the interaction of ATP with rabbit muscle phosphofructokinase. J Biol Chem. 1979 Dec 25;254(24):12289–12290.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
December 25, 1979
Volume
254
Issue
24
Start / End Page
12289 / 12290
Location
United States
Related Subject Headings
- Rabbits
- Phosphofructokinase-1
- Muscles
- Hydrogen-Ion Concentration
- Calorimetry
- Biochemistry & Molecular Biology
- Animals
- Adenosine Triphosphate
- 11 Medical and Health Sciences
- 06 Biological Sciences