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Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1.

Publication ,  Journal Article
Cantley, LC; Hammes, GG
Published in: Biochemistry
July 1975

The method of fluorescence energy transfer is used to measure the distance from the tight nucleotide binding sites to the 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole reactive sites on solubilized spinach chloroplast coupling factor 1 (CF1). The fluorescent adenine nucleotide analogs 1,N-6-ethenoadenosine diphosphate and 1,N-6-ethenoadenylyl imidodiphosphate were used as donors and 4-nitrobenzo-2-oxa-1,3-diazole bound to a tyrosine group and to an amino group were used as acceptors of energy transfer. Using three different donor-acceptor pairs, the distance measured varied from 38 to 43 A assuming both donor sites are equidistant from the acceptor site. A model is proposed for the location of the tight nucleotide binding sites and the active site on the alpha and beta subunits of CF1.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

July 1975

Volume

14

Issue

13

Start / End Page

2976 / 2981

Location

United States

Related Subject Headings

  • Protein Conformation
  • Oxadiazoles
  • Nucleotides
  • Nitro Compounds
  • Models, Chemical
  • Mathematics
  • Ligands
  • Fluorescence
  • Chloroplasts
  • Biochemistry & Molecular Biology
 

Citation

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Cantley, L. C., & Hammes, G. G. (1975). Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1. Biochemistry, 14(13), 2976–2981. https://doi.org/10.1021/bi00684a028
Cantley, L. C., and G. G. Hammes. “Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1.Biochemistry 14, no. 13 (July 1975): 2976–81. https://doi.org/10.1021/bi00684a028.
Cantley LC, Hammes GG. Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1. Biochemistry. 1975 Jul;14(13):2976–81.
Cantley, L. C., and G. G. Hammes. “Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1.Biochemistry, vol. 14, no. 13, July 1975, pp. 2976–81. Pubmed, doi:10.1021/bi00684a028.
Cantley LC, Hammes GG. Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1. Biochemistry. 1975 Jul;14(13):2976–2981.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

July 1975

Volume

14

Issue

13

Start / End Page

2976 / 2981

Location

United States

Related Subject Headings

  • Protein Conformation
  • Oxadiazoles
  • Nucleotides
  • Nitro Compounds
  • Models, Chemical
  • Mathematics
  • Ligands
  • Fluorescence
  • Chloroplasts
  • Biochemistry & Molecular Biology