Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1.

Journal Article (Journal Article)

The method of fluorescence energy transfer is used to measure the distance from the tight nucleotide binding sites to the 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole reactive sites on solubilized spinach chloroplast coupling factor 1 (CF1). The fluorescent adenine nucleotide analogs 1,N-6-ethenoadenosine diphosphate and 1,N-6-ethenoadenylyl imidodiphosphate were used as donors and 4-nitrobenzo-2-oxa-1,3-diazole bound to a tyrosine group and to an amino group were used as acceptors of energy transfer. Using three different donor-acceptor pairs, the distance measured varied from 38 to 43 A assuming both donor sites are equidistant from the acceptor site. A model is proposed for the location of the tight nucleotide binding sites and the active site on the alpha and beta subunits of CF1.

Full Text

Duke Authors

Cited Authors

  • Cantley, LC; Hammes, GG

Published Date

  • July 1975

Published In

Volume / Issue

  • 14 / 13

Start / End Page

  • 2976 - 2981

PubMed ID

  • 1148188

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00684a028


  • eng

Conference Location

  • United States