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Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA.

Publication ,  Journal Article
Holzer, KP; Liu, W; Hammes, GG
Published in: Proc Natl Acad Sci U S A
June 1989

The complete amino acid sequence of chicken liver fatty acid synthase [acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing, and thioester-hydrolyzing), EC 2.3.1.85] has been determined from the corresponding cDNA sequence. A 5.3-kilobase-pair (kbp) region of cDNA coding for chicken fatty acid synthase has been cloned and sequenced that is contiguous to the 2.3-kbp region previously sequenced [Yuan, Z., Liu, W. & Hammes, G.G. (1988) Proc. Natl. Acad. Sci. USA 85, 6328-6331]. The cDNA codes for the remaining 1677 amino acids of the previously unsequenced region of the protein. The amino acid sequence contains peptides known to be associated with the NADPH binding site of the enoylreductase active center, the acetyl/malonyltransacylase active site, the "waiting" site containing cysteine, and a pyridoxal 5'-phosphate binding site. Locations of the NADPH binding site of the beta-ketoacylreductase active site and of the dehydratase active site are proposed on the basis of protein sequence homologies to catalytic sites in other enzymes. The molecular weight of the complete polypeptide chain is 267,288. A linear functional map of the chicken fatty acid synthase derived from its primary sequence is presented.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1989

Volume

86

Issue

12

Start / End Page

4387 / 4391

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Molecular Sequence Data
  • Liver
  • Genes
  • Fatty Acid Synthases
  • DNA
  • Cloning, Molecular
  • Chickens
  • Base Sequence
 

Citation

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Holzer, K. P., Liu, W., & Hammes, G. G. (1989). Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA. Proc Natl Acad Sci U S A, 86(12), 4387–4391. https://doi.org/10.1073/pnas.86.12.4387
Holzer, K. P., W. Liu, and G. G. Hammes. “Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA.Proc Natl Acad Sci U S A 86, no. 12 (June 1989): 4387–91. https://doi.org/10.1073/pnas.86.12.4387.
Holzer KP, Liu W, Hammes GG. Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4387–91.
Holzer, K. P., et al. “Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA.Proc Natl Acad Sci U S A, vol. 86, no. 12, June 1989, pp. 4387–91. Pubmed, doi:10.1073/pnas.86.12.4387.
Holzer KP, Liu W, Hammes GG. Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4387–4391.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1989

Volume

86

Issue

12

Start / End Page

4387 / 4391

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Molecular Sequence Data
  • Liver
  • Genes
  • Fatty Acid Synthases
  • DNA
  • Cloning, Molecular
  • Chickens
  • Base Sequence