Differential requirements for segment polarity genes in wingless signaling.

Journal Article

The segment polarity genes wingless and engrailed are required throughout development of Drosophila. During early embryogenesis, these two genes are expressed in adjacent domains, in an inter-dependent way. Later, their expression is regulated by different mechanisms and becomes maintained by auto-regulation. To dissect the genetic requirements for the initial signaling between wingless and engrailed expressing cells, we have previously used a transgenic Drosophila strain that expresses wingless under the control of the heat shock promoter (HS-wg). Focusing on the later phases of wingless and engrailed regulation, we have now extended these studies, using embryos carrying various combinations of segment polarity mutations and the HS-wg transgene. We confirm some of the existing models of regulation of the expression of wingless and engrailed. In addition, we find that HS-wg embryos require engrailed for induction of ectopic endogenous wingless expression. Signaling from engrailed cells to this novel wingless expression domain is dependent on hedgehog but also on porcupine. We further demonstrate a novel requirement for hedgehog in maintenance of expression of engrailed itself.

Full Text

Duke Authors

Cited Authors

  • Noordermeer, J; Klingensmith, J; Nusse, R

Published Date

  • June 1995

Published In

Volume / Issue

  • 51 / 2-3

Start / End Page

  • 145 - 155

PubMed ID

  • 7547463

International Standard Serial Number (ISSN)

  • 0925-4773

Language

  • eng

Conference Location

  • Ireland