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The mammalian twisted gastrulation gene functions in foregut and craniofacial development.

Publication ,  Journal Article
Petryk, A; Anderson, RM; Jarcho, MP; Leaf, I; Carlson, CS; Klingensmith, J; Shawlot, W; O'Connor, MB
Published in: Dev Biol
March 15, 2004

Extracellular modulators of cell-cell signaling control numerous aspects of organismal development. The Twisted gastrulation (Twsg1) gene product is a small, secreted cysteine-rich protein that has the unusual property of being able to either enhance or inhibit signaling by the bone morphogenetic protein (BMP) subfamily of TGF-beta type factors in a context-dependent manner. In this report, we characterize the early embryonic and skeletal phenotypes associated with loss of Twsg1 function in mice. All Twsg1 mutant mice, irrespective of genetic background, exhibit deletions of neural arches in the cervical vertebrae. In a C57BL/6 background, we also observe pronounced forebrain defects including rostral truncations, holoprosencephaly, cyclopia, as well as alterations in the first branchial arch (BA1) leading to lack of jaw (agnathia). Characterization of marker expression suggests that these defects are attributable to loss of signaling from forebrain-organizing centers including Fgf8 from the anterior neural ridge (ANR) and Shh from the prechordal plate (PrCP). In addition, we find defects in the foregut endoderm and a reduction in Hex expression, which may contribute to both the forebrain and BA1 defects.

Duke Scholars

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

March 15, 2004

Volume

267

Issue

2

Start / End Page

374 / 386

Location

United States

Related Subject Headings

  • Upper Gastrointestinal Tract
  • Trans-Activators
  • Signal Transduction
  • Proteins
  • Mice, Knockout
  • Mice
  • In Situ Hybridization
  • Histological Techniques
  • Hedgehog Proteins
  • Gene Expression Regulation, Developmental
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Petryk, A., Anderson, R. M., Jarcho, M. P., Leaf, I., Carlson, C. S., Klingensmith, J., … O’Connor, M. B. (2004). The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev Biol, 267(2), 374–386. https://doi.org/10.1016/j.ydbio.2003.11.015
Petryk, Anna, Ryan M. Anderson, Michael P. Jarcho, Irina Leaf, Cathy S. Carlson, John Klingensmith, William Shawlot, and Michael B. O’Connor. “The mammalian twisted gastrulation gene functions in foregut and craniofacial development.Dev Biol 267, no. 2 (March 15, 2004): 374–86. https://doi.org/10.1016/j.ydbio.2003.11.015.
Petryk A, Anderson RM, Jarcho MP, Leaf I, Carlson CS, Klingensmith J, et al. The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev Biol. 2004 Mar 15;267(2):374–86.
Petryk, Anna, et al. “The mammalian twisted gastrulation gene functions in foregut and craniofacial development.Dev Biol, vol. 267, no. 2, Mar. 2004, pp. 374–86. Pubmed, doi:10.1016/j.ydbio.2003.11.015.
Petryk A, Anderson RM, Jarcho MP, Leaf I, Carlson CS, Klingensmith J, Shawlot W, O’Connor MB. The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev Biol. 2004 Mar 15;267(2):374–386.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

March 15, 2004

Volume

267

Issue

2

Start / End Page

374 / 386

Location

United States

Related Subject Headings

  • Upper Gastrointestinal Tract
  • Trans-Activators
  • Signal Transduction
  • Proteins
  • Mice, Knockout
  • Mice
  • In Situ Hybridization
  • Histological Techniques
  • Hedgehog Proteins
  • Gene Expression Regulation, Developmental