Role of sugars and organic acids in regulating the concentration and activity of the alternative oxidase in Poa annua roots.

Journal Article (Journal Article)

Detached roots of Poa annua were used to study alternative oxidase protein expression upon the addition of sucrose, glucose, fructose, inositol, mannitol, citrate or malate, at a concentration of 1 or 10 mM for 24 h. After 24 h the capacity of cytochrome c oxidase was decreased equally in all treatments. Only citrate induced the expression of the alternative oxidase, especially at a concentration of 1 mM (15-fold). The activity of the alternative pathway (measured with the (18)O-fractionation technique) was not affected by the addition of sucrose for 24 h as compared with time zero. However, after the addition of citrate or mannitol the activity of the alternative pathway decreased to almost zero. The discrepancy between the large increase in alternative oxidase protein concentration when citrate was applied and the concomitant decrease in alternative pathway activity is discussed.

Full Text

Duke Authors

Cited Authors

  • Millenaar, FF; Gonzalez-Meler, MA; Siedow, JN; Wagner, AM; Lambers, H

Published Date

  • May 2002

Published In

Volume / Issue

  • 53 / 371

Start / End Page

  • 1081 - 1088

PubMed ID

  • 11971919

Pubmed Central ID

  • 11971919

Electronic International Standard Serial Number (EISSN)

  • 1460-2431

International Standard Serial Number (ISSN)

  • 0022-0957

Digital Object Identifier (DOI)

  • 10.1093/jexbot/53.371.1081


  • eng