Quantitation of in vivo measurements with carbon fiber microelectrodes.


Journal Article

Fast-scan cyclic voltammetry (FSCV) at carbon fiber disk microelectrodes and quantitative microdialysis were used to measure striatal concentration changes of N-acetyl-p-aminophenol (APAP, acetaminophen) following an intraperitoneal injection of 75 mg/kg APAP in rats. The goal of this work was to determine which in vitro calibration procedure, precalibration or postcalibration, gave the most accurate results when using carbon fiber microelectrodes in vivo. Voltammetric detection of APAP in vivo was complicated with normal electrodes by interference from pH changes. An electrode treatment was used to minimize electrode sensitivity to pH and this allowed successful APAP detection. In vitro calibrations of the treated carbon fiber disk microelectrodes before and after the in vivo experiment were used to calculate APAP concentration changes measured in vivo and compared to microdialysis results. The maximal striatal APAP concentration determined by microdialysis, adjusted for in vitro recovery, was 23.1 microM. The electrochemical results were approximately two times greater (postcalibration) or smaller (precalibration) than the microdialysis result.

Full Text

Duke Authors

Cited Authors

  • Logman, MJ; Budygin, EA; Gainetdinov, RR; Wightman, RM

Published Date

  • February 2000

Published In

Volume / Issue

  • 95 / 2

Start / End Page

  • 95 - 102

PubMed ID

  • 10752479

Pubmed Central ID

  • 10752479

Electronic International Standard Serial Number (EISSN)

  • 1872-678X

International Standard Serial Number (ISSN)

  • 0165-0270

Digital Object Identifier (DOI)

  • 10.1016/s0165-0270(99)00155-7


  • eng