A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides.
Here we report on the application of a solid-solid (SS) sample preparation protocol for the MALDI analysis of peptides and multicomponent peptide mixtures. Our results with a series of model peptides indicate that a SS MALDI sample preparation protocol is useful for the analysis of peptides in the 1-3 kDa mass range. MALDI mass spectra recorded for peptides in this size range using a SS sample preparation were of a quality comparable to spectra recorded using a conventional dried-droplet (DD) sample preparation. Our results with several model peptide mixtures indicate that one advantage of a SS sample preparation protocol for the MALDI analysis of peptides is that it can significantly reduce signal suppression effects in multicomponent mixtures. MALDI results obtained using a SS sample preparation protocol are also more reproducible than results obtained using a conventional DD sample preparation protocol.
Duke Scholars
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Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Peptides
- Molecular Sequence Data
- Analytical Chemistry
- Amino Acid Sequence
- 4004 Chemical engineering
- 3401 Analytical chemistry
- 3205 Medical biochemistry and metabolomics
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Peptides
- Molecular Sequence Data
- Analytical Chemistry
- Amino Acid Sequence
- 4004 Chemical engineering
- 3401 Analytical chemistry
- 3205 Medical biochemistry and metabolomics
- 0399 Other Chemical Sciences
- 0301 Analytical Chemistry