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A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides.

Publication ,  Journal Article
Wang, MZ; Fitzgerald, MC
Published in: Analytical chemistry
February 2001

Here we report on the application of a solid-solid (SS) sample preparation protocol for the MALDI analysis of peptides and multicomponent peptide mixtures. Our results with a series of model peptides indicate that a SS MALDI sample preparation protocol is useful for the analysis of peptides in the 1-3 kDa mass range. MALDI mass spectra recorded for peptides in this size range using a SS sample preparation were of a quality comparable to spectra recorded using a conventional dried-droplet (DD) sample preparation. Our results with several model peptide mixtures indicate that one advantage of a SS sample preparation protocol for the MALDI analysis of peptides is that it can significantly reduce signal suppression effects in multicomponent mixtures. MALDI results obtained using a SS sample preparation protocol are also more reproducible than results obtained using a conventional DD sample preparation protocol.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2001

Volume

73

Issue

3

Start / End Page

625 / 631

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Peptides
  • Molecular Sequence Data
  • Analytical Chemistry
  • Amino Acid Sequence
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry
 

Citation

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Wang, M. Z., & Fitzgerald, M. C. (2001). A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides. Analytical Chemistry, 73(3), 625–631. https://doi.org/10.1021/ac0009090
Wang, M. Z., and M. C. Fitzgerald. “A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides.Analytical Chemistry 73, no. 3 (February 2001): 625–31. https://doi.org/10.1021/ac0009090.
Wang, M. Z., and M. C. Fitzgerald. “A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides.Analytical Chemistry, vol. 73, no. 3, Feb. 2001, pp. 625–31. Epmc, doi:10.1021/ac0009090.
Wang MZ, Fitzgerald MC. A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides. Analytical chemistry. 2001 Feb;73(3):625–631.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

February 2001

Volume

73

Issue

3

Start / End Page

625 / 631

Related Subject Headings

  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Peptides
  • Molecular Sequence Data
  • Analytical Chemistry
  • Amino Acid Sequence
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 3205 Medical biochemistry and metabolomics
  • 0399 Other Chemical Sciences
  • 0301 Analytical Chemistry