Direct characterization of solid phase resin-bound molecules by mass spectrometry

Journal Article (Journal Article)

In this work we demonstrate that analytes, covalently linked to a polymeric support through a photolabile linker, can be directly analyzed by matrix-assisted laser desorption/ionization (MALDI). Mass spectral analysis is performed in a single step requiring no pretreatment of the sample to induce cleavage from the support. Our results show that the UV laser light in the MALDI experiment can be used to simultaneously promote an analyte's photolytic cleavage from a solid support and its gas phase ionization for subsequent mass spectral analysis. In this manner, MALDI facilitates the dissociation and identification of resin-bound analytes in a single analytical procedure without the need for any prior chemical treatment.

Full Text

Duke Authors

Cited Authors

  • Fitzgerald, MC; Harris, K; Shevlin, CG; Siuzdak, G

Published Date

  • April 23, 1996

Published In

Volume / Issue

  • 6 / 8

Start / End Page

  • 979 - 982

International Standard Serial Number (ISSN)

  • 0960-894X

Digital Object Identifier (DOI)

  • 10.1016/0960-894X(96)00152-7

Citation Source

  • Scopus