Mass spectrometry as a readout of protein structure and function.


Journal Article (Review)

Proteins have evolved to carry out very specific functions within the cell by interacting with a diverse set of biomolecules. Understanding how a protein's higher order structure relates to its function is important for defining the molecular basis of these interactions. In recent years, mass spectrometry has become an important tool for dissecting protein structure and function. Using electrospray ionization (ESI)- and matrix-assisted laser desorption/ionization (MALDI)-based approaches, it has been possible to monitor protein folding, characterize noncovalent protein complexes, and assess the contribution of individual amino acid residues to a protein's function. Here, it is our goal to summarize these approaches and highlight recent, biologically relevant applications where mass spectrometry has provided unique insight into the mysteries of protein structure and function.

Full Text

Duke Authors

Cited Authors

  • Winston, RL; Fitzgerald, MC

Published Date

  • July 1997

Published In

Volume / Issue

  • 16 / 4

Start / End Page

  • 165 - 179

PubMed ID

  • 9449832

Pubmed Central ID

  • 9449832

Electronic International Standard Serial Number (EISSN)

  • 1098-2787

International Standard Serial Number (ISSN)

  • 0277-7037

Digital Object Identifier (DOI)

  • 10.1002/(sici)1098-2787(1997)16:4<165::aid-mas1>;2-f


  • eng