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Mass spectrometry as a readout of protein structure and function.

Publication ,  Journal Article
Winston, RL; Fitzgerald, MC
Published in: Mass spectrometry reviews
July 1997

Proteins have evolved to carry out very specific functions within the cell by interacting with a diverse set of biomolecules. Understanding how a protein's higher order structure relates to its function is important for defining the molecular basis of these interactions. In recent years, mass spectrometry has become an important tool for dissecting protein structure and function. Using electrospray ionization (ESI)- and matrix-assisted laser desorption/ionization (MALDI)-based approaches, it has been possible to monitor protein folding, characterize noncovalent protein complexes, and assess the contribution of individual amino acid residues to a protein's function. Here, it is our goal to summarize these approaches and highlight recent, biologically relevant applications where mass spectrometry has provided unique insight into the mysteries of protein structure and function.

Duke Scholars

Published In

Mass spectrometry reviews

DOI

EISSN

1098-2787

ISSN

0277-7037

Publication Date

July 1997

Volume

16

Issue

4

Start / End Page

165 / 179

Related Subject Headings

  • Proto-Oncogene Proteins c-crk
  • Proto-Oncogene Proteins
  • Proteins
  • Protein Folding
  • Peptide Mapping
  • Molecular Sequence Data
  • Mass Spectrometry
  • Animals
  • Analytical Chemistry
  • Amino Acid Sequence
 

Citation

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Winston, R. L., & Fitzgerald, M. C. (1997). Mass spectrometry as a readout of protein structure and function. Mass Spectrometry Reviews, 16(4), 165–179. https://doi.org/10.1002/(sici)1098-2787(1997)16:4<165::aid-mas1>3.0.co;2-f
Winston, R. L., and M. C. Fitzgerald. “Mass spectrometry as a readout of protein structure and function.Mass Spectrometry Reviews 16, no. 4 (July 1997): 165–79. https://doi.org/10.1002/(sici)1098-2787(1997)16:4<165::aid-mas1>3.0.co;2-f.
Winston RL, Fitzgerald MC. Mass spectrometry as a readout of protein structure and function. Mass spectrometry reviews. 1997 Jul;16(4):165–79.
Winston, R. L., and M. C. Fitzgerald. “Mass spectrometry as a readout of protein structure and function.Mass Spectrometry Reviews, vol. 16, no. 4, July 1997, pp. 165–79. Epmc, doi:10.1002/(sici)1098-2787(1997)16:4<165::aid-mas1>3.0.co;2-f.
Winston RL, Fitzgerald MC. Mass spectrometry as a readout of protein structure and function. Mass spectrometry reviews. 1997 Jul;16(4):165–179.
Journal cover image

Published In

Mass spectrometry reviews

DOI

EISSN

1098-2787

ISSN

0277-7037

Publication Date

July 1997

Volume

16

Issue

4

Start / End Page

165 / 179

Related Subject Headings

  • Proto-Oncogene Proteins c-crk
  • Proto-Oncogene Proteins
  • Proteins
  • Protein Folding
  • Peptide Mapping
  • Molecular Sequence Data
  • Mass Spectrometry
  • Animals
  • Analytical Chemistry
  • Amino Acid Sequence