On the origins of congenic MATalpha and MATa strains of the pathogenic yeast Cryptococcus neoformans.

Journal Article (Journal Article;Review)

The basidiomycetous yeast Cryptococcus neoformans infects humans and causes a meningoencephalitis that is uniformly fatal if untreated. The organism has a defined sexual cycle involving mating of haploid MATa and MATalpha strains, gene disruption by transformation and homologous recombination is now readily accomplished, and robust animal models for infection have been well established. In addition, a pair of congenic MATalpha and MATa haploid strains have been constructed that permit detailed studies on physiology and virulence by classical genetic approaches. These strains represent a valuable resource for further studies in this organism, and the genomic sequence of one of these strains, JEC21 (=B-4500), was recently chosen to be sequenced by an international consortium. Because of the importance of these strains for genetic studies in C. neoformans and the fact that the genomic sequence of one of these strains is in progress, we review here how these congenic strains were originally constructed.

Full Text

Duke Authors

Cited Authors

  • Heitman, J; Allen, B; Alspaugh, JA; Kwon-Chung, KJ

Published Date

  • October 1999

Published In

Volume / Issue

  • 28 / 1

Start / End Page

  • 1 - 5

PubMed ID

  • 10512666

International Standard Serial Number (ISSN)

  • 1087-1845

Digital Object Identifier (DOI)

  • 10.1006/fgbi.1999.1155


  • eng

Conference Location

  • United States