Ras1 controls pheromone expression and response during mating in Cryptococcus neoformans.


Journal Article

The Cryptococcus neoformans Ras1 signal transduction pathway controls mating, hyphal differentiation, and the ability of this opportunistic human fungal pathogen to grow at elevated temperatures. To further elucidate how Ras1 signals in this organism, the RAS1 gene was disrupted in the congenic serotype D strain background. Genetic epistasis experiments indicated that Ras1 regulates the mating response through the MAP kinase/pheromone response pathway. In fact, Ras1 is required for the transcriptional induction of elements of the pheromone response pathway. However, the ability of C. neoformans Ras1 to allow growth at 37 degrees C is mediated by a separate signaling pathway. Therefore a single Ras protein may differentially activate distinct downstream targets in response to different signals within the same organism. This conserved signaling motif has been coopted in C. neoformans to regulate mating and morphogenesis in addition to being required for its pathogenic potential.

Full Text

Duke Authors

Cited Authors

  • Waugh, MS; Vallim, MA; Heitman, J; Alspaugh, JA

Published Date

  • February 2003

Published In

Volume / Issue

  • 38 / 1

Start / End Page

  • 110 - 121

PubMed ID

  • 12553941

Pubmed Central ID

  • 12553941

International Standard Serial Number (ISSN)

  • 1087-1845

Digital Object Identifier (DOI)

  • 10.1016/s1087-1845(02)00518-2


  • eng

Conference Location

  • United States