Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands.

Published

Journal Article

The kinetics of interaction between TCR and MHC-peptide show a general relationship between affinity and the biological response, but the reported kinetic differences between antigenic and antagonistic peptides are very small. Here, we show a remarkable difference in the kinetics of TCR interactions with strong agonist ligands at 37 degrees C compared to 25 degrees C. This difference is not seen with antagonist/positive selecting ligands. The interaction at 37 degrees C shows biphasic binding kinetics best described by a model of TCR dimerization. The altered kinetics greatly increase the stability of complexes with agonist ligands, accounting for the large differences in biological response compared to other ligands. Thus, there may be an allosteric, as well as a kinetic, component to the discrimination between agonists and antagonists.

Full Text

Duke Authors

Cited Authors

  • Alam, SM; Davies, GM; Lin, CM; Zal, T; Nasholds, W; Jameson, SC; Hogquist, KA; Gascoigne, NR; Travers, PJ

Published Date

  • February 1999

Published In

Volume / Issue

  • 10 / 2

Start / End Page

  • 227 - 237

PubMed ID

  • 10072075

Pubmed Central ID

  • 10072075

International Standard Serial Number (ISSN)

  • 1074-7613

Language

  • eng

Conference Location

  • United States