Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands.
Journal Article (Journal Article)
The kinetics of interaction between TCR and MHC-peptide show a general relationship between affinity and the biological response, but the reported kinetic differences between antigenic and antagonistic peptides are very small. Here, we show a remarkable difference in the kinetics of TCR interactions with strong agonist ligands at 37 degrees C compared to 25 degrees C. This difference is not seen with antagonist/positive selecting ligands. The interaction at 37 degrees C shows biphasic binding kinetics best described by a model of TCR dimerization. The altered kinetics greatly increase the stability of complexes with agonist ligands, accounting for the large differences in biological response compared to other ligands. Thus, there may be an allosteric, as well as a kinetic, component to the discrimination between agonists and antagonists.
Full Text
Duke Authors
Cited Authors
- Alam, SM; Davies, GM; Lin, CM; Zal, T; Nasholds, W; Jameson, SC; Hogquist, KA; Gascoigne, NR; Travers, PJ
Published Date
- February 1999
Published In
Volume / Issue
- 10 / 2
Start / End Page
- 227 - 237
PubMed ID
- 10072075
International Standard Serial Number (ISSN)
- 1074-7613
Digital Object Identifier (DOI)
- 10.1016/s1074-7613(00)80023-0
Language
- eng
Conference Location
- United States