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Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands.

Publication ,  Journal Article
Alam, SM; Davies, GM; Lin, CM; Zal, T; Nasholds, W; Jameson, SC; Hogquist, KA; Gascoigne, NR; Travers, PJ
Published in: Immunity
February 1999

The kinetics of interaction between TCR and MHC-peptide show a general relationship between affinity and the biological response, but the reported kinetic differences between antigenic and antagonistic peptides are very small. Here, we show a remarkable difference in the kinetics of TCR interactions with strong agonist ligands at 37 degrees C compared to 25 degrees C. This difference is not seen with antagonist/positive selecting ligands. The interaction at 37 degrees C shows biphasic binding kinetics best described by a model of TCR dimerization. The altered kinetics greatly increase the stability of complexes with agonist ligands, accounting for the large differences in biological response compared to other ligands. Thus, there may be an allosteric, as well as a kinetic, component to the discrimination between agonists and antagonists.

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Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

February 1999

Volume

10

Issue

2

Start / End Page

227 / 237

Location

United States

Related Subject Headings

  • Temperature
  • Receptors, Antigen, T-Cell
  • Peptides
  • Peptide Fragments
  • Ovalbumin
  • Major Histocompatibility Complex
  • Ligands
  • Kinetics
  • Immunology
  • H-2 Antigens
 

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Alam, S. M., Davies, G. M., Lin, C. M., Zal, T., Nasholds, W., Jameson, S. C., … Travers, P. J. (1999). Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. Immunity, 10(2), 227–237. https://doi.org/10.1016/s1074-7613(00)80023-0
Alam, S. M., G. M. Davies, C. M. Lin, T. Zal, W. Nasholds, S. C. Jameson, K. A. Hogquist, N. R. Gascoigne, and P. J. Travers. “Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands.Immunity 10, no. 2 (February 1999): 227–37. https://doi.org/10.1016/s1074-7613(00)80023-0.
Alam SM, Davies GM, Lin CM, Zal T, Nasholds W, Jameson SC, et al. Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. Immunity. 1999 Feb;10(2):227–37.
Alam, S. M., et al. “Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands.Immunity, vol. 10, no. 2, Feb. 1999, pp. 227–37. Pubmed, doi:10.1016/s1074-7613(00)80023-0.
Alam SM, Davies GM, Lin CM, Zal T, Nasholds W, Jameson SC, Hogquist KA, Gascoigne NR, Travers PJ. Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. Immunity. 1999 Feb;10(2):227–237.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

February 1999

Volume

10

Issue

2

Start / End Page

227 / 237

Location

United States

Related Subject Headings

  • Temperature
  • Receptors, Antigen, T-Cell
  • Peptides
  • Peptide Fragments
  • Ovalbumin
  • Major Histocompatibility Complex
  • Ligands
  • Kinetics
  • Immunology
  • H-2 Antigens