Aneuploid subpopulations in tumour-invaded lymph nodes from breast cancer patients.

Journal Article (Journal Article)

Fresh, paired primary tumours and lymph node metastases from breast cancer patients were compared by DNA flow cytometry. Although 65% of primary tumours were aneuploid, the detection of aneuploid peaks in corresponding nodal metastases was rare (only 6 cases out of 25) in single-parameter DNA analysis. Detection of aneuploid subpopulations in lymph nodes was greatly improved in dual-parameter DNA analysis using an anticytokeratin (CK) antibody which allowed ploidy determination on CK+ epithelial cells alone. Examination of 12 lymph nodes for CK+ cells revealed the presence of both diploid and aneuploid tumour cells in tumour invaded nodes. In patients with multiploid primary tumours, a subpopulation of the primary aneuploid cells was dominant in the nodal metastases. This suggests that aneuploidy is an integral property of metastatic cells and that within a primary tumour a subpopulation may have a higher metastatic potential.

Full Text

Duke Authors

Cited Authors

  • Alam, SM; Whitford, P; Cushley, W; George, WD; Campbell, AM

Published Date

  • 1992

Published In

Volume / Issue

  • 28 / 2-3

Start / End Page

  • 357 - 362

PubMed ID

  • 1375482

Pubmed Central ID

  • 1375482

International Standard Serial Number (ISSN)

  • 0959-8049

Digital Object Identifier (DOI)

  • 10.1016/s0959-8049(05)80053-x

Language

  • eng

Conference Location

  • England