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Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.

Publication ,  Journal Article
Kuehn, MJ; Schekman, R; Ljungdahl, PO
Published in: J Cell Biol
November 1996

In S. cerevisiae lacking SHR3, amino acid permeases specifically accumulate in membranes of the endoplasmic reticulum (ER) and fail to be transported to the plasma membrane. We examined the requirements of transport of the permeases from the ER to the Golgi in vitro. Addition of soluble COPII components (Sec23/24p, Sec13/31p, and Sar1p) to yeast membrane preparations generated vesicles containing the general amino acid permease. Gap1p, and the histidine permease, Hip1p. Shr3p was required for the packaging of Gap1p and Hip1p but was not itself incorporated into transport vesicles. In contrast, the packaging of the plasma membrane ATPase, Pma1p, and the soluble yeast pheromone precursor, glycosylated pro alpha factor, was independent of Shr3p. In addition, we show that integral membrane and soluble cargo colocalize in transport vesicles, indicating that different types of cargo are not segregated at an early step in secretion. Our data suggest that specific ancillary proteins in the ER membrane recruit subsets of integral membrane protein cargo into COPII transport vesicles.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

November 1996

Volume

135

Issue

3

Start / End Page

585 / 595

Location

United States

Related Subject Headings

  • Vesicular Transport Proteins
  • Spheroplasts
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Proton-Translocating ATPases
  • Protein Precursors
  • Peptides
  • Nuclear Pore Complex Proteins
  • Monomeric GTP-Binding Proteins
  • Microsomes
 

Citation

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Kuehn, M. J., Schekman, R., & Ljungdahl, P. O. (1996). Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro. J Cell Biol, 135(3), 585–595. https://doi.org/10.1083/jcb.135.3.585
Kuehn, M. J., R. Schekman, and P. O. Ljungdahl. “Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.J Cell Biol 135, no. 3 (November 1996): 585–95. https://doi.org/10.1083/jcb.135.3.585.
Kuehn, M. J., et al. “Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.J Cell Biol, vol. 135, no. 3, Nov. 1996, pp. 585–95. Pubmed, doi:10.1083/jcb.135.3.585.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

November 1996

Volume

135

Issue

3

Start / End Page

585 / 595

Location

United States

Related Subject Headings

  • Vesicular Transport Proteins
  • Spheroplasts
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Proton-Translocating ATPases
  • Protein Precursors
  • Peptides
  • Nuclear Pore Complex Proteins
  • Monomeric GTP-Binding Proteins
  • Microsomes