Differential dye-ligand chromatography as a general purification protocol for 2-keto-3-deoxy-6-phosphogluconate aldolases

Published

Journal Article

2-Keto-3-deoxy-6-phosphogluconate aldolases (KDPG aldolases, EC 4.1.2.14) from four sources, Esherichia coli, Pseudomonas putida, Pseudomonas saccharophila, and Zymononas mobilis, were purified from 17- to 48- fold by differential dye-ligand chromatography (DD-L chromatography). This technique, using Procion Navy H-ER (150 grain) as a negative column and Procion Yellow MX-GR as a positive column supported on Sepharose CL-4B, represents a general methodology for the rapid, facile purification of KDPG aldolases from disparate microbial sources. © 1994.

Full Text

Duke Authors

Cited Authors

  • Shelton, MC; Toone, EJ

Published Date

  • January 1, 1995

Published In

Volume / Issue

  • 6 / 1

Start / End Page

  • 207 - 211

Electronic International Standard Serial Number (EISSN)

  • 1362-511X

International Standard Serial Number (ISSN)

  • 0957-4166

Digital Object Identifier (DOI)

  • 10.1016/0957-4166(94)00376-M

Citation Source

  • Scopus