Differential dye-ligand chromatography as a general purification protocol for 2-keto-3-deoxy-6-phosphogluconate aldolases
Journal Article (Journal Article)
2-Keto-3-deoxy-6-phosphogluconate aldolases (KDPG aldolases, EC 4.1.2.14) from four sources, Esherichia coli, Pseudomonas putida, Pseudomonas saccharophila, and Zymononas mobilis, were purified from 17- to 48- fold by differential dye-ligand chromatography (DD-L chromatography). This technique, using Procion Navy H-ER (150 grain) as a negative column and Procion Yellow MX-GR as a positive column supported on Sepharose CL-4B, represents a general methodology for the rapid, facile purification of KDPG aldolases from disparate microbial sources. © 1994.
Full Text
Duke Authors
Cited Authors
- Shelton, MC; Toone, EJ
Published Date
- January 1, 1995
Published In
Volume / Issue
- 6 / 1
Start / End Page
- 207 - 211
Electronic International Standard Serial Number (EISSN)
- 1362-511X
International Standard Serial Number (ISSN)
- 0957-4166
Digital Object Identifier (DOI)
- 10.1016/0957-4166(94)00376-M
Citation Source
- Scopus