The synthetic utility of KDPGal aldolase


Journal Article

We describe the isolation and purification of KDPGal aldolase from two sources. To facilitate this isolation, a facile five-step chemical synthesis of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) (1) from commercially available D-galactono-1,4-lactone (3) is described. The key steps are the β- elimination of the protected aldonolactone 4 to furnish the desired 2,3- unsaturated ketone 5 and selective phosphorylation of the primary hydroxyl moiety of the diol 7. The purity of the KDPGal sample was > 95% as determined by enzymatic assay using crude KDPGal aldolase from Pseudomonas saccharophila. An initial substrate specificity and pH-activity profile of KDPGal aldolase are described.

Full Text

Duke Authors

Cited Authors

  • Cotterill, IC; Henderson, DP; Shelton, MC; Toone, EJ

Published Date

  • September 15, 1998

Published In

Volume / Issue

  • 5 / 1-4

Start / End Page

  • 103 - 111

International Standard Serial Number (ISSN)

  • 1381-1177

Digital Object Identifier (DOI)

  • 10.1016/S1381-1177(98)00087-3

Citation Source

  • Scopus