Induction of micronuclei by X-radiation in human, mouse and rat peripheral blood lymphocytes.

Published

Journal Article

We compared the radiosensitivity of human, rat and mouse peripheral blood lymphocytes (PBLs) by analyzing micronuclei (MN) in cytochalasin B-induced binucleated (BN) cells. For each species and dose 4-ml aliquots of whole blood were X-irradiated to obtain doses of 38, 75, 150 or 300 cGy. Controls were sham-irradiated. After exposure to X-rays, mononuclear leukocytes were isolated using density gradients and cultured in RPMI 1640 medium containing phytohemagglutinin to stimulate mitogenesis. At 21 h cytochalasin B was added to produce BN PBLs, and all cultures were harvested at 52 h post-initiation using a cytocentrifuge. Significant dose-dependent increases in the percentage of micronucleated cells and the number of MN per BN cell were observed in all three species. The linear-quadratic regression curves for the total percentage of micronucleated cells for the three species were similar; however, the curve for the mouse PBLs had a larger quadratic component than either of the curves for the rat or human PBLs. Although the correlation between the percentage of cells with MN and those with chromosome aberrations was high (r2 greater than 0.95), the mouse and rat PBLs were over twice as efficient as human PBLs in forming MN from presumed acentric fragments. These data indicate that the induction of MN in BN cells following ionizing radiation is similar in human, rat and mouse PBLs, but care must be taken in using the MN results to predict frequencies of cells with chromosomal aberrations.

Full Text

Duke Authors

Cited Authors

  • Erexson, GL; Kligerman, AD; Bryant, MF; Sontag, MR; Halperin, EC

Published Date

  • October 1991

Published In

Volume / Issue

  • 253 / 2

Start / End Page

  • 193 - 198

PubMed ID

  • 1922145

Pubmed Central ID

  • 1922145

International Standard Serial Number (ISSN)

  • 0027-5107

Digital Object Identifier (DOI)

  • 10.1016/0165-1161(91)90132-r

Language

  • eng

Conference Location

  • Netherlands