Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases.
Publication
, Journal Article
Dobrikov, MI; Grady, KM; Shaw, BR
Published in: Nucleosides, nucleotides & nucleic acids
March 2003
A series of 2'-deoxynucleoside 5'-triphosphates (dNTPs) and their alpha-P-thio or alpha-P-borano analogues, i.e., (Sp-dNTPalphaS), (Rp-dNTPalphaB) and (Sp-dNTPalphaB) were studied as substrates for DNA dependent DNA polymerases and HIV-1 reverse transcriptase (RT). For HIV-1 RT the Rp-dNTPalphaB isomers are 1.2-fold better substrates than natural dNTPs. For DNA polymerases their efficiencies of incorporation are 3-fold (Klenow, Sequenase) and 5-fold (Taq) lower than for dNTPs. Thus, introduction of the alpha-boranophosphate group into dNTPs increases their selectivity to HIV-1 RT relative to bacterial DNA polymerases.
Duke Scholars
Published In
Nucleosides, nucleotides & nucleic acids
DOI
EISSN
1532-2335
ISSN
1525-7770
Publication Date
March 2003
Volume
22
Issue
3
Start / End Page
275 / 282
Related Subject Headings
- Taq Polymerase
- Stereoisomerism
- Organic Chemistry
- Kinetics
- HIV Reverse Transcriptase
- Deoxycytosine Nucleotides
- DNA-Directed DNA Polymerase
- DNA Polymerase I
- DNA
- Boron Compounds
Citation
APA
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ICMJE
MLA
NLM
Dobrikov, M. I., Grady, K. M., & Shaw, B. R. (2003). Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases. Nucleosides, Nucleotides & Nucleic Acids, 22(3), 275–282. https://doi.org/10.1081/ncn-120021427
Dobrikov, Mikhail I., Kristen M. Grady, and Barbara Ramsay Shaw. “Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases.” Nucleosides, Nucleotides & Nucleic Acids 22, no. 3 (March 2003): 275–82. https://doi.org/10.1081/ncn-120021427.
Dobrikov MI, Grady KM, Shaw BR. Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases. Nucleosides, nucleotides & nucleic acids. 2003 Mar;22(3):275–82.
Dobrikov, Mikhail I., et al. “Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases.” Nucleosides, Nucleotides & Nucleic Acids, vol. 22, no. 3, Mar. 2003, pp. 275–82. Epmc, doi:10.1081/ncn-120021427.
Dobrikov MI, Grady KM, Shaw BR. Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases. Nucleosides, nucleotides & nucleic acids. 2003 Mar;22(3):275–282.
Published In
Nucleosides, nucleotides & nucleic acids
DOI
EISSN
1532-2335
ISSN
1525-7770
Publication Date
March 2003
Volume
22
Issue
3
Start / End Page
275 / 282
Related Subject Headings
- Taq Polymerase
- Stereoisomerism
- Organic Chemistry
- Kinetics
- HIV Reverse Transcriptase
- Deoxycytosine Nucleotides
- DNA-Directed DNA Polymerase
- DNA Polymerase I
- DNA
- Boron Compounds