Introduction of the alpha-P-borano-group into deoxynucleoside triphosphates increases their selectivity to HIV-1 reverse transcriptase relative to DNA polymerases.

Published

Journal Article

A series of 2'-deoxynucleoside 5'-triphosphates (dNTPs) and their alpha-P-thio or alpha-P-borano analogues, i.e., (Sp-dNTPalphaS), (Rp-dNTPalphaB) and (Sp-dNTPalphaB) were studied as substrates for DNA dependent DNA polymerases and HIV-1 reverse transcriptase (RT). For HIV-1 RT the Rp-dNTPalphaB isomers are 1.2-fold better substrates than natural dNTPs. For DNA polymerases their efficiencies of incorporation are 3-fold (Klenow, Sequenase) and 5-fold (Taq) lower than for dNTPs. Thus, introduction of the alpha-boranophosphate group into dNTPs increases their selectivity to HIV-1 RT relative to bacterial DNA polymerases.

Full Text

Duke Authors

Cited Authors

  • Dobrikov, MI; Grady, KM; Shaw, BR

Published Date

  • March 2003

Published In

Volume / Issue

  • 22 / 3

Start / End Page

  • 275 - 282

PubMed ID

  • 12816386

Pubmed Central ID

  • 12816386

Electronic International Standard Serial Number (EISSN)

  • 1532-2335

International Standard Serial Number (ISSN)

  • 1525-7770

Digital Object Identifier (DOI)

  • 10.1081/ncn-120021427

Language

  • eng