RNase H activation by stereoregular boranophosphate oligonucleotide.

Journal Article (Journal Article)

A stereoregular all-(Sp)-boranophosphate oligodeoxyribonucleotide (BH3(-)-ODN) 15-mer was synthesized using an enzymatic approach. The BH3(-)-ODN formed a hybrid with the complementary RNA 15-mer and induced RNase H hydrolysis of the RNA strand at ODN concentrations as low as 10 nM at 37 degrees C, but with a lower efficiency than that of its natural phosphodiester analogue.

Full Text

Duke Authors

Cited Authors

  • Wang, X; Dobrikov, M; Sergueev, D; Shaw, BR

Published Date

  • May 2003

Published In

Volume / Issue

  • 22 / 5-8

Start / End Page

  • 1151 - 1153

PubMed ID

  • 14565367

Electronic International Standard Serial Number (EISSN)

  • 1532-2335

International Standard Serial Number (ISSN)

  • 1525-7770

Digital Object Identifier (DOI)

  • 10.1081/ncn-120022823


  • eng