Incorporation of (alpha-P-borano)-2',3'-dideoxycytidine 5'-triphosphate into DNA by drug-resistant MMLV reverse transcriptase and Taq DNA polymerase.

Published

Journal Article

The Rp-stereoisomer of 5'-(alpha-P-borano)triphosphates of 2'-deoxycytidine (Rp-dCTPalphaB) and 2',3'-dideoxycytidine (Rp-ddCTPalphaB) were synthesized. Their steady-state kinetics of incorporation by ddNTP-resistant enzymes, e.g., MMLV reverse transcriptase (RT) and Taq DNA polymerase, were investigated and compared with incorporation of dCTP and ddCTP. The alpha-boranophosphate substitution in ddCTP results in a 28-fold increase in efficiency of incorporation of the Rp-ddCTPalphaB isomer by MMLV RT, yet has minimal effect on the efficiency of incorporation by Taq DNA polymerase.

Full Text

Duke Authors

Cited Authors

  • Dobrikov, MI; Sergueeva, ZA; Shaw, BR

Published Date

  • May 2003

Published In

Volume / Issue

  • 22 / 5-8

Start / End Page

  • 1651 - 1655

PubMed ID

  • 14565487

Pubmed Central ID

  • 14565487

Electronic International Standard Serial Number (EISSN)

  • 1532-2335

International Standard Serial Number (ISSN)

  • 1525-7770

Digital Object Identifier (DOI)

  • 10.1081/ncn-120023091

Language

  • eng