Skip to main content
Journal cover image

Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin

Publication ,  Journal Article
Plunkett, SE; Chao, JL; Tague, TJ; Palmer, RA
Published in: Applied Spectroscopy
June 1, 1995

The kinetics of protein response and of CO recombination after photolysis of the Fe-CO bond in carbonmonoxymyoglobin have been monitored via time-resolved step-scan FT-IR absorption difference spectroscopy in D2O solution. Although the initial photodissociation is too fast to observe with currently available FT-IR instrumentation, we have been able to correlate the CO recombination kinetics with protein secondary structural changes via changes in the amide I band of the polypeptide chain with microsecond time resolution. The spectral and kinetic data corroborate and confirm previously published single-frequency infrared studies. This is the first application of time-resolved step-scan FT-IR spectroscopy in the absorbance difference mode to study the photodynamics of an aqueous protein solution at room temperature. This work also demonstrates the potential of the technique for the sub-microsecond kinetic analysis of other biological molecules of interest.

Duke Scholars

Published In

Applied Spectroscopy

DOI

EISSN

1943-3530

ISSN

0003-7028

Publication Date

June 1, 1995

Volume

49

Issue

6

Start / End Page

702 / 708

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Plunkett, S. E., Chao, J. L., Tague, T. J., & Palmer, R. A. (1995). Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin. Applied Spectroscopy, 49(6), 702–708. https://doi.org/10.1366/0003702953964633
Plunkett, S. E., J. L. Chao, T. J. Tague, and R. A. Palmer. “Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin.” Applied Spectroscopy 49, no. 6 (June 1, 1995): 702–8. https://doi.org/10.1366/0003702953964633.
Plunkett SE, Chao JL, Tague TJ, Palmer RA. Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin. Applied Spectroscopy. 1995 Jun 1;49(6):702–8.
Plunkett, S. E., et al. “Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin.” Applied Spectroscopy, vol. 49, no. 6, June 1995, pp. 702–08. Scopus, doi:10.1366/0003702953964633.
Plunkett SE, Chao JL, Tague TJ, Palmer RA. Time-Resolved Step-Scan FT-IR Spectroscopy of the Photodynamics of Carbonmonoxymyoglobin. Applied Spectroscopy. 1995 Jun 1;49(6):702–708.
Journal cover image

Published In

Applied Spectroscopy

DOI

EISSN

1943-3530

ISSN

0003-7028

Publication Date

June 1, 1995

Volume

49

Issue

6

Start / End Page

702 / 708

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry