Hydrodynamic evidence in support of spacer regions in chromatin.

Journal Article (Journal Article)

Quasi-elastic light scattering and sedimentation velocity methods were used to study the hydrodynamic properties of purified dimer subunits obtained from partial digestion of chicken erythrocyte chromatin with staphylococcal nuclease. The experimental value of 1.87 +/- 0.08 X 10(-7) gram per second for the friction factor of these dimer subunits in low ionic strength buffer cannot be reasonably interpreted in terms of a contiguous sphere model. Analysis by means of an equivalent dimer method suggests that the spacer region accounts for a maximum of 19 percent of the friction properties of the dimer.

Full Text

Duke Authors

Cited Authors

  • Schmitz, KS; Shaw, BR

Published Date

  • August 1, 1977

Published In

Volume / Issue

  • 197 / 4304

Start / End Page

  • 661 - 663

PubMed ID

  • 877579

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.877579


  • eng