Hydrodynamic evidence in support of spacer regions in chromatin.
Journal Article (Journal Article)
Quasi-elastic light scattering and sedimentation velocity methods were used to study the hydrodynamic properties of purified dimer subunits obtained from partial digestion of chicken erythrocyte chromatin with staphylococcal nuclease. The experimental value of 1.87 +/- 0.08 X 10(-7) gram per second for the friction factor of these dimer subunits in low ionic strength buffer cannot be reasonably interpreted in terms of a contiguous sphere model. Analysis by means of an equivalent dimer method suggests that the spacer region accounts for a maximum of 19 percent of the friction properties of the dimer.
Full Text
Duke Authors
Cited Authors
- Schmitz, KS; Shaw, BR
Published Date
- August 1, 1977
Published In
Volume / Issue
- 197 / 4304
Start / End Page
- 661 - 663
PubMed ID
- 877579
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.877579
Language
- eng