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NHERF-1 is required for renal adaptation to a low-phosphate diet.

Publication ,  Journal Article
Weinman, EJ; Boddeti, A; Cunningham, R; Akom, M; Wang, F; Wang, Y; Liu, J; Steplock, D; Shenolikar, S; Wade, JB
Published in: Am J Physiol Renal Physiol
December 2003

The sodium-dependent renal phosphate transporter (Npt2, Na-Pi IIa) is the major regulated phosphate transporter in the renal proximal convoluted tubule. Npt2 associates with a number of PDZ-containing proteins including Na+H+ exchanger regulatory factor-1 (NHERF-1). To determine whether NHERF-1 is involved in the acute regulation of phosphate transport, wild-type and NHERF-1 (-/-) mice were stabilized on a high-phosphate diet and then acutely changed to a low-phosphate diet. At 24 h after the change to a low-phosphate diet, there was a significant decrease in the urinary excretion of phosphate in both groups but the urinary excretion of phosphate in NHERF-1 (-/-) mice was significantly higher than in wild-type animals (1,097 +/- 356 vs. 255 +/- 54 ng/min, P < 0.05). Renal mRNA levels and total cellular Npt2 protein did not differ between the animal groups or in response to the changes in diet. Renal brush-border membrane (BBM) expression of Npt2 protein, however, was lower in NHERF-1 (-/-) mice compared with wild-type. In addition, with both the high- and low-phosphate diets, there was increased detection of Npt2 in submicrovillar domains that were particularly prominent in NHERF-1 (-/-) mice compared with wild-type animals. On the other hand, a change from a low-phosphate diet to a high-phosphate diet was associated with a similar increase in the urinary excretion of phosphate in wild-type and NHERF-1 (-/-) animals. These experiments demonstrate that full renal adaptation to a low-phosphate diet requires NHERF-1, which serves to increase BBM expression of Npt2.

Duke Scholars

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

December 2003

Volume

285

Issue

6

Start / End Page

F1225 / F1232

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Symporters
  • Sodium-Phosphate Cotransporter Proteins, Type III
  • Sodium-Phosphate Cotransporter Proteins, Type I
  • Sodium-Phosphate Cotransporter Proteins
  • Sodium-Hydrogen Exchangers
  • RNA, Messenger
  • Phosphoproteins
  • Phosphates
  • Mice, Mutant Strains
 

Citation

APA
Chicago
ICMJE
MLA
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Weinman, E. J., Boddeti, A., Cunningham, R., Akom, M., Wang, F., Wang, Y., … Wade, J. B. (2003). NHERF-1 is required for renal adaptation to a low-phosphate diet. Am J Physiol Renal Physiol, 285(6), F1225–F1232. https://doi.org/10.1152/ajprenal.00215.2003
Weinman, Edward J., Anuradha Boddeti, Rochelle Cunningham, Michael Akom, Fengying Wang, Yu Wang, Jie Liu, Deborah Steplock, Shirish Shenolikar, and James B. Wade. “NHERF-1 is required for renal adaptation to a low-phosphate diet.Am J Physiol Renal Physiol 285, no. 6 (December 2003): F1225–32. https://doi.org/10.1152/ajprenal.00215.2003.
Weinman EJ, Boddeti A, Cunningham R, Akom M, Wang F, Wang Y, et al. NHERF-1 is required for renal adaptation to a low-phosphate diet. Am J Physiol Renal Physiol. 2003 Dec;285(6):F1225–32.
Weinman, Edward J., et al. “NHERF-1 is required for renal adaptation to a low-phosphate diet.Am J Physiol Renal Physiol, vol. 285, no. 6, Dec. 2003, pp. F1225–32. Pubmed, doi:10.1152/ajprenal.00215.2003.
Weinman EJ, Boddeti A, Cunningham R, Akom M, Wang F, Wang Y, Liu J, Steplock D, Shenolikar S, Wade JB. NHERF-1 is required for renal adaptation to a low-phosphate diet. Am J Physiol Renal Physiol. 2003 Dec;285(6):F1225–F1232.

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

December 2003

Volume

285

Issue

6

Start / End Page

F1225 / F1232

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Symporters
  • Sodium-Phosphate Cotransporter Proteins, Type III
  • Sodium-Phosphate Cotransporter Proteins, Type I
  • Sodium-Phosphate Cotransporter Proteins
  • Sodium-Hydrogen Exchangers
  • RNA, Messenger
  • Phosphoproteins
  • Phosphates
  • Mice, Mutant Strains