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Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor.

Publication ,  Journal Article
Weinman, EJ; Steplock, D; Zhang, X; Akhter, S; Shenolikar, S
Published in: Biochim Biophys Acta
October 6, 1999

The Na/H exchanger regulatory factor (NHE-RF) was first identified as a co-factor for cAMP dependent protein kinase regulation of the rabbit epithelial Na/H exchanger. Subsequently, this protein which contains two PDZ motifs, was shown to interact with multiple cellular targets. To understand more fully the function of NHE-RF and its regulation, we have cloned the full-length cDNA for mouse NHE-RF and a portion of the mouse gene containing the promoter elements. NHE-RF cDNA, isolated from a mouse kidney cDNA library, predicted a polypeptide of 356 amino acids that shares striking sequence conservation within the two PDZ domains and in-vitro phosphorylation sites with the human and rat homologs. The nucleotide sequence 5' of the transcription start site, identified by primer extension analysis, was highly 'GC' rich and lacked canonical TATA or CAAT sequences. Using a luciferase reporter construct, deletion analyses localized the critical segment for gene expression in mouse medullary thick ascending limb cells to 114 bp 5' of the transcription start site. Although NHE-RF has been recently identified as an estrogen-inducible gene, the lack of an estrogen-response element in the mouse NHE-RF 5'-non-coding-sequence and the inability to demonstrate estrogen stimulation of reporter gene expression in MCF-7 cells suggests a non-conventional or indirect mechanism for NHE-RF regulation by estrogen.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

October 6, 1999

Volume

1447

Issue

1

Start / End Page

71 / 76

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Sodium-Hydrogen Exchangers
  • Sequence Alignment
  • Restriction Mapping
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Molecular Sequence Data
  • Mice
  • Kidney
 

Citation

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Weinman, E. J., Steplock, D., Zhang, X., Akhter, S., & Shenolikar, S. (1999). Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Biochim Biophys Acta, 1447(1), 71–76. https://doi.org/10.1016/s0167-4781(99)00100-1
Weinman, E. J., D. Steplock, X. Zhang, S. Akhter, and S. Shenolikar. “Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor.Biochim Biophys Acta 1447, no. 1 (October 6, 1999): 71–76. https://doi.org/10.1016/s0167-4781(99)00100-1.
Weinman EJ, Steplock D, Zhang X, Akhter S, Shenolikar S. Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Biochim Biophys Acta. 1999 Oct 6;1447(1):71–6.
Weinman, E. J., et al. “Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor.Biochim Biophys Acta, vol. 1447, no. 1, Oct. 1999, pp. 71–76. Pubmed, doi:10.1016/s0167-4781(99)00100-1.
Weinman EJ, Steplock D, Zhang X, Akhter S, Shenolikar S. Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Biochim Biophys Acta. 1999 Oct 6;1447(1):71–76.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

October 6, 1999

Volume

1447

Issue

1

Start / End Page

71 / 76

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Sodium-Hydrogen Exchangers
  • Sequence Alignment
  • Restriction Mapping
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Molecular Sequence Data
  • Mice
  • Kidney