N-terminal PDZ domain is required for NHERF dimerization.
Publication
, Journal Article
Shenolikar, S; Minkoff, CM; Steplock, DA; Evangelista, C; Liu, M; Weinman, EJ
Published in: FEBS Lett
February 2, 2001
NHERF, a 55 kDa PDZ-containing protein, binds receptors and ion transporters to mediate signal transduction at the plasma membrane. Recombinant NHERF demonstrated an apparent size of 150 kDa on gel filtration, which could be reduced to approximately 55 kDa by protein denaturing agents, consistent with the formation of NHERF dimers. Biosensor studies established the time- and concentration-dependent dimerization of NHERF. Overlays of recombinant NHERF fragments suggested that NHERF dimerization was principally mediated by the N-terminal PDZ-I domain. In PS120 cells, reversible protein phosphorylation modulated NHERF dimerization and suggested a role for NHERF dimers in hormonal signaling.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
February 2, 2001
Volume
489
Issue
2-3
Start / End Page
233 / 236
Location
England
Related Subject Headings
- Sodium-Hydrogen Exchangers
- Recombinant Proteins
- Rabbits
- Protein Structure, Tertiary
- Protein Binding
- Phosphorylation
- Phosphoproteins
- Peptide Fragments
- Dimerization
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Shenolikar, S., Minkoff, C. M., Steplock, D. A., Evangelista, C., Liu, M., & Weinman, E. J. (2001). N-terminal PDZ domain is required for NHERF dimerization. FEBS Lett, 489(2–3), 233–236. https://doi.org/10.1016/s0014-5793(01)02109-3
Shenolikar, S., C. M. Minkoff, D. A. Steplock, C. Evangelista, M. Liu, and E. J. Weinman. “N-terminal PDZ domain is required for NHERF dimerization.” FEBS Lett 489, no. 2–3 (February 2, 2001): 233–36. https://doi.org/10.1016/s0014-5793(01)02109-3.
Shenolikar S, Minkoff CM, Steplock DA, Evangelista C, Liu M, Weinman EJ. N-terminal PDZ domain is required for NHERF dimerization. FEBS Lett. 2001 Feb 2;489(2–3):233–6.
Shenolikar, S., et al. “N-terminal PDZ domain is required for NHERF dimerization.” FEBS Lett, vol. 489, no. 2–3, Feb. 2001, pp. 233–36. Pubmed, doi:10.1016/s0014-5793(01)02109-3.
Shenolikar S, Minkoff CM, Steplock DA, Evangelista C, Liu M, Weinman EJ. N-terminal PDZ domain is required for NHERF dimerization. FEBS Lett. 2001 Feb 2;489(2–3):233–236.
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
February 2, 2001
Volume
489
Issue
2-3
Start / End Page
233 / 236
Location
England
Related Subject Headings
- Sodium-Hydrogen Exchangers
- Recombinant Proteins
- Rabbits
- Protein Structure, Tertiary
- Protein Binding
- Phosphorylation
- Phosphoproteins
- Peptide Fragments
- Dimerization
- Cell Line