N-terminal PDZ domain is required for NHERF dimerization.

Published

Journal Article

NHERF, a 55 kDa PDZ-containing protein, binds receptors and ion transporters to mediate signal transduction at the plasma membrane. Recombinant NHERF demonstrated an apparent size of 150 kDa on gel filtration, which could be reduced to approximately 55 kDa by protein denaturing agents, consistent with the formation of NHERF dimers. Biosensor studies established the time- and concentration-dependent dimerization of NHERF. Overlays of recombinant NHERF fragments suggested that NHERF dimerization was principally mediated by the N-terminal PDZ-I domain. In PS120 cells, reversible protein phosphorylation modulated NHERF dimerization and suggested a role for NHERF dimers in hormonal signaling.

Full Text

Duke Authors

Cited Authors

  • Shenolikar, S; Minkoff, CM; Steplock, DA; Evangelista, C; Liu, M; Weinman, EJ

Published Date

  • February 2, 2001

Published In

Volume / Issue

  • 489 / 2-3

Start / End Page

  • 233 - 236

PubMed ID

  • 11165256

Pubmed Central ID

  • 11165256

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/s0014-5793(01)02109-3

Language

  • eng

Conference Location

  • England