Effect of limited trypsin digestion on the renal Na+-H+ exchanger and its regulation by cAMP-dependent protein kinase.

Published

Journal Article

The Na+-H+ exchanger from solubilized rabbit renal brush border membranes is inhibited by cAMP-dependent protein kinase (PKA) mediated protein phosphorylation. To characterize this inhibitory response and its sensitivity to limited proteolysis, the activity of the transporter was assayed after reconstitution of the proteins into artificial lipid vesicles. Limited trypsin digestion increased the basal rate of proton gradient-stimulated, amiloride-inhibitable sodium uptake in reconstituted proteoliposomes and blocked the inhibitory response to PKA-mediated protein phosphorylation. To determine if the inhibitory response to PKA-mediated protein phosphorylation could be restored to the trypsin-treated solubilized proteins, nontrypsinized solubilized brush border membrane proteins were separated by column chromatography. The addition of small molecular weight polypeptides, fractionated on Superose-12 FPLC (Ve = 0.7), to trypsinized solubilized brush border membrane proteins restored the inhibitory response to PKA-mediated protein phosphorylation. Similarly, the addition of the 0.1 M NaCl fraction from an anion exchange column, Mono Q-FPLC, also restored the inhibitory response to PKA. Both protein fractions contained a common 42-43 kDa protein which was preferentially phosphorylated by PKA. These results indicate that limited trypsin digestion dissociates the activity of the renal Na+-H+ exchanger from its regulation by PKA. It is suggested that trypsin cleaves an inhibitory component of the transporter and that this component is the site of PKA-mediated regulation. Phosphoprotein analysis of fractions that restored PKA regulation raises the possibility that a polypeptide of 42-43 kDa is involved in the inhibition of the renal Na+-H+ exchanger by PKA-mediated protein phosphorylation.

Full Text

Duke Authors

Cited Authors

  • Weinman, EJ; Dubinsky, WP; Dinh, Q; Steplock, D; Shenolikar, S

Published Date

  • August 1, 1989

Published In

Volume / Issue

  • 109 / 3

Start / End Page

  • 233 - 241

PubMed ID

  • 2552124

Pubmed Central ID

  • 2552124

International Standard Serial Number (ISSN)

  • 0022-2631

Digital Object Identifier (DOI)

  • 10.1007/bf01870280

Language

  • eng

Conference Location

  • United States