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Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices.

Publication ,  Journal Article
Shenolikar, S; Karbon, EW; Enna, SJ
Published in: Biochem Biophys Res Commun
August 29, 1986

The effect of phorbol esters on cyclic AMP production in rat cerebral cortical slices was studied using a prelabelling technique to measure cyclic nucleotide accumulation. Cholera toxin-stimulated cyclic AMP accumulation was enhanced approximately 2-fold by phorbol 12-myristate, 13-acetate (PMA) which alone had no effect on cyclic AMP production. The augmentation by PMA was maximal within the first hour of incubation, decreasing progressively thereafter. Protein kinase C activity was decreased 80-90% during a 3 hr exposure to PMA, as was 3H-phorbol 12,13-dibutyrate binding. Both phosphatidyl serine and arachidonic acid were found to enhance protein kinase C activity in a concentration-dependent manner, an effect that was attenuated by prolonged incubation of the brain tissue with PMA. The results indicate that exposure of brain slices to phorbol esters causes a down-regulation of rat brain protein kinase C, and that this modification corresponds with a decrease in the ability of PMA to augment cyclic AMP production, suggesting a functional relationship between the two systems in rat brain.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 29, 1986

Volume

139

Issue

1

Start / End Page

251 / 258

Location

United States

Related Subject Headings

  • Tetradecanoylphorbol Acetate
  • Rats
  • Protein Kinase C
  • Phosphatidylserines
  • Phorbol Esters
  • Phorbol 12,13-Dibutyrate
  • In Vitro Techniques
  • Cyclic AMP
  • Cholera Toxin
  • Cerebral Cortex
 

Citation

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Shenolikar, S., Karbon, E. W., & Enna, S. J. (1986). Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices. Biochem Biophys Res Commun, 139(1), 251–258. https://doi.org/10.1016/s0006-291x(86)80106-1
Shenolikar, S., E. W. Karbon, and S. J. Enna. “Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices.Biochem Biophys Res Commun 139, no. 1 (August 29, 1986): 251–58. https://doi.org/10.1016/s0006-291x(86)80106-1.
Shenolikar S, Karbon EW, Enna SJ. Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices. Biochem Biophys Res Commun. 1986 Aug 29;139(1):251–8.
Shenolikar, S., et al. “Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices.Biochem Biophys Res Commun, vol. 139, no. 1, Aug. 1986, pp. 251–58. Pubmed, doi:10.1016/s0006-291x(86)80106-1.
Shenolikar S, Karbon EW, Enna SJ. Phorbol esters down-regulate protein kinase C in rat brain cerebral cortical slices. Biochem Biophys Res Commun. 1986 Aug 29;139(1):251–258.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 29, 1986

Volume

139

Issue

1

Start / End Page

251 / 258

Location

United States

Related Subject Headings

  • Tetradecanoylphorbol Acetate
  • Rats
  • Protein Kinase C
  • Phosphatidylserines
  • Phorbol Esters
  • Phorbol 12,13-Dibutyrate
  • In Vitro Techniques
  • Cyclic AMP
  • Cholera Toxin
  • Cerebral Cortex