Regulation of synaptic strength by protein phosphatase 1.

Published

Journal Article

We investigated the role of postsynaptic protein phosphatase 1 (PP1) in regulating synaptic strength by loading CA1 pyramidal cells either with peptides that disrupt PP1 binding to synaptic targeting proteins or with active PP1. The peptides blocked synaptically evoked LTD but had no effect on basal synaptic currents mediated by either AMPA or NMDA receptors. They did, however, cause an increase in synaptic strength following the induction of LTD. Similarly, PP1 had no effect on basal synaptic strength but enhanced LTD. In cultured neurons, synaptic activation of NMDA receptors increased the proportion of PP1 localized to synapses. These results suggest that PP1 does not significantly regulate basal synaptic strength. Appropriate NMDA receptor activation, however, allows PP1 to gain access to synaptic substrates and be recruited to synapses where its activity is necessary for sustaining LTD.

Full Text

Duke Authors

Cited Authors

  • Morishita, W; Connor, JH; Xia, H; Quinlan, EM; Shenolikar, S; Malenka, RC

Published Date

  • December 20, 2001

Published In

Volume / Issue

  • 32 / 6

Start / End Page

  • 1133 - 1148

PubMed ID

  • 11754843

Pubmed Central ID

  • 11754843

International Standard Serial Number (ISSN)

  • 0896-6273

Language

  • eng

Conference Location

  • United States