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Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana.

Publication ,  Journal Article
Silverstone, AL; Chang, C; Krol, E; Sun, TP
Published in: The Plant journal : for cell and molecular biology
July 1997

The GA1 gene of Arabidopsis thaliana encodes ent-kaurene synthase A (KSA), which catalyzes the first committed step in the biosynthetic pathway of the plant hormone gibberellin (GA). Its location in the GA biosynthetic pathway has led to speculation that KSA regulation is one of the controlling steps. However, because KSA activity is so low that it is only measurable in Arabidopsis siliques, GA1 promoter-GUS reporter gene fusions and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were used to examine the expression pattern of GA1. The results from this study indicate that GA1 gene expression is highly regulated during growth and development, and it is restricted to specific cell types at the sites of expression. GA1 promoter activity is highest in rapidly growing tissues, e.g. shoot apicas, root tips, developing flowers and seeds. It is also active in the vascular tissue of some non-growing organs, such as expanded leaves, suggesting that these leaves may be a site of GA synthesis for transport to other organs. It was also found that the first one or two introns in the GA1 gene are required for proper expression. Because of the high degree of regulation, GA1 may act as a gatekeeper, controlling the flow of metabolites into the GA biosynthetic pathway, while the levels of specific bioactive GAs are controlled by other downstream steps.

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Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

July 1997

Volume

12

Issue

1

Start / End Page

9 / 19

Related Subject Headings

  • Transferases
  • Transcription, Genetic
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Plant Proteins
  • Plant Biology & Botany
  • Introns
  • Gibberellins
 

Citation

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Silverstone, A. L., Chang, C., Krol, E., & Sun, T. P. (1997). Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana. The Plant Journal : For Cell and Molecular Biology, 12(1), 9–19. https://doi.org/10.1046/j.1365-313x.1997.12010009.x
Silverstone, A. L., C. Chang, E. Krol, and T. P. Sun. “Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana.The Plant Journal : For Cell and Molecular Biology 12, no. 1 (July 1997): 9–19. https://doi.org/10.1046/j.1365-313x.1997.12010009.x.
Silverstone AL, Chang C, Krol E, Sun TP. Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana. The Plant journal : for cell and molecular biology. 1997 Jul;12(1):9–19.
Silverstone, A. L., et al. “Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana.The Plant Journal : For Cell and Molecular Biology, vol. 12, no. 1, July 1997, pp. 9–19. Epmc, doi:10.1046/j.1365-313x.1997.12010009.x.
Silverstone AL, Chang C, Krol E, Sun TP. Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana. The Plant journal : for cell and molecular biology. 1997 Jul;12(1):9–19.
Journal cover image

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

July 1997

Volume

12

Issue

1

Start / End Page

9 / 19

Related Subject Headings

  • Transferases
  • Transcription, Genetic
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Plant Proteins
  • Plant Biology & Botany
  • Introns
  • Gibberellins