Skip to main content

Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1.

Publication ,  Journal Article
Tcherepanova, I; Puigserver, P; Norris, JD; Spiegelman, BM; McDonnell, DP
Published in: J Biol Chem
May 26, 2000

A transcriptional coactivator of the peroxisome proliferator-activated receptor-gamma (PPARgamma), PPARgamma-coactivator-1(PGC-1) interacts in a constitutive manner with the hinge domain of PPARgamma and enhances its transcriptional activity. In this study we demonstrate that PGC-1 is a coactivator of estrogen receptor-alpha (ERalpha)-dependent transcriptional activity. However the mechanism by which PGC-1 interacts with ERalpha is different from that of PPARgamma. Specifically, it was determined that the carboxyl terminus of PGC-1 interacts in a ligand-independent manner with the ERalpha hinge domain. In addition, an LXXLL motif within the amino terminus of PGC-1 was shown to interact in an agonist-dependent manner with the AF2 domain within the carboxyl terminus of ERalpha. The ability of PGC-1 to associate with and potentiate the transcriptional activity of an ERalpha-AF2 mutant that is unable to interact with the p160 class of coactivators suggests that this coactivator may have a unique role in estrogen signaling. It is concluded from these studies that PGC-1 is a bona fide ERalpha coactivator, which may serve as a convergence point between PPARgamma and ERalpha signaling.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 26, 2000

Volume

275

Issue

21

Start / End Page

16302 / 16308

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Receptors, Estrogen
  • Receptors, Cytoplasmic and Nuclear
  • Mutation
  • Humans
  • Estrogen Receptor alpha
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tcherepanova, I., Puigserver, P., Norris, J. D., Spiegelman, B. M., & McDonnell, D. P. (2000). Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1. J Biol Chem, 275(21), 16302–16308. https://doi.org/10.1074/jbc.M001364200
Tcherepanova, I., P. Puigserver, J. D. Norris, B. M. Spiegelman, and D. P. McDonnell. “Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1.J Biol Chem 275, no. 21 (May 26, 2000): 16302–8. https://doi.org/10.1074/jbc.M001364200.
Tcherepanova I, Puigserver P, Norris JD, Spiegelman BM, McDonnell DP. Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1. J Biol Chem. 2000 May 26;275(21):16302–8.
Tcherepanova, I., et al. “Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1.J Biol Chem, vol. 275, no. 21, May 2000, pp. 16302–08. Pubmed, doi:10.1074/jbc.M001364200.
Tcherepanova I, Puigserver P, Norris JD, Spiegelman BM, McDonnell DP. Modulation of estrogen receptor-alpha transcriptional activity by the coactivator PGC-1. J Biol Chem. 2000 May 26;275(21):16302–16308.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 26, 2000

Volume

275

Issue

21

Start / End Page

16302 / 16308

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Receptors, Estrogen
  • Receptors, Cytoplasmic and Nuclear
  • Mutation
  • Humans
  • Estrogen Receptor alpha