Cloning and functional analysis of TipE, a novel membrane protein that enhances Drosophila para sodium channel function.

Published

Journal Article

Voltage-dependent sodium channels are involved in the initiation and propagation of action potentials in many excitable cells. Here we report that tipE, a gene defined by a temperature-sensitive paralytic mutation in Drosophila, encodes a novel integral membrane protein that dramatically stimulates functional expression in Xenopus oocytes of the Drosophila sodium channel alpha subunit encoded by the paralytic (para) locus. Using a heat shock promoter to control tipE+ gene expression in transgenic flies, we demonstrate that tipE+ gene expression is required during pupal development to rescue adult paralysis. In addition, we demonstrate a role for the tipE gene product in adults.

Full Text

Cited Authors

  • Feng, G; Deák, P; Chopra, M; Hall, LM

Published Date

  • September 1995

Published In

Volume / Issue

  • 82 / 6

Start / End Page

  • 1001 - 1011

PubMed ID

  • 7553842

Pubmed Central ID

  • 7553842

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(95)90279-1

Language

  • eng