Generation of transgenic mice.

Published

Journal Article

In this post-genomic era, emphasis has shifted from identifying genes to understanding the physiological functions of gene products and their implications in human diseases. The use of transgenic mice is one of the key approaches in elucidating gene function and regulation. Transgenic mice have wide applications in biomedical research. These include (1) gain-of-function studies by overexpressing a protein in a tissue-specific manner; (2) loss-of-function studies by overexpressing a dominant-negative construct; (3) mapping functional domains by expressing various mutated constructs in a null (knockout mice) background; (4) mapping regulatory elements using a reporter gene; and (5) labeling subsets of cells with fluorescent proteins for live in vivo imaging of cell structure and function. In this chapter, we provide overviews as well as detailed protocols for each step involved in the generation of transgenic mice, including the selection of regulatory elements, purification of DNA, obtaining fertilized eggs, pronuclear injection, and genotyping of transgenic mice.

Full Text

Cited Authors

  • Feng, G; Lu, J; Gross, J

Published Date

  • January 2004

Published In

Volume / Issue

  • 99 /

Start / End Page

  • 255 - 267

PubMed ID

  • 15131343

Pubmed Central ID

  • 15131343

Electronic International Standard Serial Number (EISSN)

  • 1940-6037

International Standard Serial Number (ISSN)

  • 1543-1894

Digital Object Identifier (DOI)

  • 10.1385/1-59259-770-x:255

Language

  • eng