Labeling neurons in vivo for morphological and functional studies.


Journal Article (Review)

Increasingly sophisticated strategies for labeling cells in vivo are providing unprecedented opportunities to study neurons in living animals. Transgenic expression of genetically encoded reporters enables us to monitor changes in neuronal activity in response to sensory stimuli, and the labeling of single neurons with fluorescent proteins allows the dynamics of neuronal connectivity to be observed in transgenic animals over periods ranging from minutes to months. Advances in transient labeling techniques such as viral infection and electroporation provide a rapid means by which to analyze neuronal gene function in vivo. These new approaches to labeling, manipulating and imaging neurons in intact organisms are transforming the way in which the nervous system is studied.

Full Text

Cited Authors

  • Young, P; Feng, G

Published Date

  • October 2004

Published In

Volume / Issue

  • 14 / 5

Start / End Page

  • 642 - 646

PubMed ID

  • 15464899

Pubmed Central ID

  • 15464899

Electronic International Standard Serial Number (EISSN)

  • 1873-6882

International Standard Serial Number (ISSN)

  • 0959-4388

Digital Object Identifier (DOI)

  • 10.1016/j.conb.2004.08.007


  • eng