Identification of a copper transporter family in Arabidopsis thaliana.

Published

Journal Article

Despite copper ions being crucial in proteins participating in plant processes such as electron transport, free-radical elimination and hormone perception and signaling, very little is known about copper inward transport across plant membranes. In this work, a five-member family (COPT1-5) of putative Arabidopsis copper transporters is described. We ascertain the ability of these proteins to functionally complement and transport copper in the corresponding Saccharomyces cerevisiae high-affinity copper transport mutant. The specific expression pattern of the Arabidopsis COPT1-5 mRNA in different tissues was analyzed by RT-PCR. Although all members are ubiquitously expressed, differences in their relative abundance in roots, leaves, stem and flowers have been observed. Moreover, steady-state COPT1 and COPT2 mRNA levels, the members that are most efficacious in complementing the S. cerevisiae high-affinity copper transport mutant, are down-regulated under copper excess, consistent with a role for these proteins in copper transport in Arabidopsis cells.

Full Text

Duke Authors

Cited Authors

  • Sancenón, V; Puig, S; Mira, H; Thiele, DJ; Peñarrubia, L

Published Date

  • March 2003

Published In

Volume / Issue

  • 51 / 4

Start / End Page

  • 577 - 587

PubMed ID

  • 12650623

Pubmed Central ID

  • 12650623

Electronic International Standard Serial Number (EISSN)

  • 1573-5028

International Standard Serial Number (ISSN)

  • 0167-4412

Digital Object Identifier (DOI)

  • 10.1023/a:1022345507112

Language

  • eng