Uncoating of clathrin-coated vesicles in presynaptic terminals: roles for Hsc70 and auxilin.


Journal Article

We have examined the roles of Hsc70 and auxilin in the uncoating of clathrin-coated vesicles (CCVs) during neuronal endocytosis. We identified two peptides that inhibit the ability of Hsc70 and auxilin to uncoat CCVs in vitro. When injected into nerve terminals, these peptides inhibited both synaptic transmission and CCV uncoating. Mutation of a conserved HPD motif within the J domain of auxilin prevented binding to Hsc70 in vitro and injecting this mutant protein inhibited CCV uncoating in vivo, demonstrating that the interaction of auxilin with Hsc70 is critical for CCV uncoating. These studies establish that auxilin and Hsc70 participate in synaptic vesicle recycling in neurons and that an interaction between these proteins is required for CCV uncoating.

Full Text

Cited Authors

  • Morgan, JR; Prasad, K; Jin, S; Augustine, GJ; Lafer, EM

Published Date

  • October 1, 2001

Published In

Volume / Issue

  • 32 / 2

Start / End Page

  • 289 - 300

PubMed ID

  • 11683998

Pubmed Central ID

  • 11683998

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/s0896-6273(01)00467-6


  • eng