SNAP-mediated protein-protein interactions essential for neurotransmitter release.

Published

Journal Article

The constitutive fusion of transport vesicles with intracellular membranes requires soluble proteins called SNAPs. Certain presynaptic proteins implicated in synaptic vesicle exocytosis also bind SNAPs, suggesting that SNAPs participate in the calcium-regulated membrane fusion events mediating neurotransmitter release. Here we show that injection of recombinant SNAPs into the giant synapse of squid enhances transmitter release. Conversely, injection of peptides designed to mimic the sites at which SNAP interacts with its binding partners inhibits transmitter release downstream of synaptic vesicle docking. A SNAP-dependent protein complex must therefore mediate transmitter release, showing that transmitter release shares a common molecular mechanism with constitutive membrane fusion.

Full Text

Cited Authors

  • DeBello, WM; O'Connor, V; Dresbach, T; Whiteheart, SW; Wang, SS; Schweizer, FE; Betz, H; Rothman, JE; Augustine, GJ

Published Date

  • February 1995

Published In

Volume / Issue

  • 373 / 6515

Start / End Page

  • 626 - 630

PubMed ID

  • 7854421

Pubmed Central ID

  • 7854421

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/373626a0

Language

  • eng