A post-docking role for synaptobrevin in synaptic vesicle fusion.

Published

Journal Article

We have used the squid giant synapse to determine the role of synaptobrevin, integral membrane proteins of small synaptic vesicles, in neurotransmitter release. The sequence of squid synaptobrevin, deduced by cDNA cloning, is 65%-68% identical to mammalian isoforms and includes the conserved cleavage site for tetanus and botulinum B toxins. Injection of either toxin into squid nerve terminals caused a slow, irreversible inhibition of release without affecting the Ca2+ signal which triggers release. Microinjection of a recombinant protein corresponding to the cytoplasmic domain of synaptobrevin produced a more rapid and reversible inhibition of release, whereas two smaller peptide fragments were without effect. Electron microscopy of tetanus-injected terminals revealed an increased number of both docked and undocked synaptic vesicles. These data indicate that synaptobrevin participates in neurotransmitter release at a step between vesicle docking and fusion.

Full Text

Cited Authors

  • Hunt, JM; Bommert, K; Charlton, MP; Kistner, A; Habermann, E; Augustine, GJ; Betz, H

Published Date

  • June 1, 1994

Published In

Volume / Issue

  • 12 / 6

Start / End Page

  • 1269 - 1279

PubMed ID

  • 8011337

Pubmed Central ID

  • 8011337

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/0896-6273(94)90443-x

Language

  • eng