Chromosomal organization of rRNA operons in Bacillus subtilis.

Journal Article (Journal Article)

Integrative mapping with vectors containing ribosomal DNA sequences were used to complete the mapping of the 10 rRNA gene sets in the endospore forming bacterium Bacillus subtilis. Southern hybridizations allowed the assignment of nine operons to distinct BclI restriction fragments and their genetic locus identified by transductional crosses. Nine of the ten rRNA gene sets are located between 0 and 70 degrees on the genomic map. In the region surrounding cysA14, two sets of closely spaced tandem clusters are present. The first (rrnJ and rrnW) is located between purA16 and cysA14 closely linked to the latter; the second (rrnI, rrnH and rrnG) previously mapped within this area is located between attSPO2 and glpT6. The operons at or near the origin of replication (rrnO,rrnA and rrnJ,rrnW) represent "hot spots" of plasmid insertion.

Full Text

Duke Authors

Cited Authors

  • Jarvis, ED; Widom, RL; LaFauci, G; Setoguchi, Y; Richter, IR; Rudner, R

Published Date

  • November 1988

Published In

Volume / Issue

  • 120 / 3

Start / End Page

  • 625 - 635

PubMed ID

  • 2465199

Pubmed Central ID

  • PMC1203540

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/120.3.625


  • eng

Conference Location

  • United States