Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.

Journal Article (Journal Article)

Integrable plasmids pGR102 and pWR103 containing ribosomal sequences from within the transcriptional units for 16S and 23S were used to transform Bacillus subtilis. To date, these plasmids integrated into 7 of 10 known rrn operons. Two such events occurred at unassigned operons, revealing a close linkage of the CAT gene of the plasmid to pha-1 situated between dal-1 and purB33 for rrnE and to thiA78 situated between glyB133 and re-12 for rrnD. All seven integration events that led to the loss of unique ribosomal BclI fragments can now be assigned to known rrn operons.

Full Text

Duke Authors

Cited Authors

  • LaFauci, G; Widom, RL; Eisner, RL; Jarvis, ED; Rudner, R

Published Date

  • January 1, 1986

Published In

Volume / Issue

  • 165 / 1

Start / End Page

  • 204 - 214

PubMed ID

  • 3079748

Pubmed Central ID

  • PMC214390

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.165.1.204-214.1986


  • eng

Conference Location

  • United States