Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.
Publication
, Journal Article
LaFauci, G; Widom, RL; Eisner, RL; Jarvis, ED; Rudner, R
Published in: J Bacteriol
January 1986
Integrable plasmids pGR102 and pWR103 containing ribosomal sequences from within the transcriptional units for 16S and 23S were used to transform Bacillus subtilis. To date, these plasmids integrated into 7 of 10 known rrn operons. Two such events occurred at unassigned operons, revealing a close linkage of the CAT gene of the plasmid to pha-1 situated between dal-1 and purB33 for rrnE and to thiA78 situated between glyB133 and re-12 for rrnD. All seven integration events that led to the loss of unique ribosomal BclI fragments can now be assigned to known rrn operons.
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Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
January 1986
Volume
165
Issue
1
Start / End Page
204 / 214
Location
United States
Related Subject Headings
- Transformation, Bacterial
- RNA, Ribosomal
- Plasmids
- Operon
- Nucleic Acid Hybridization
- Microbiology
- Genes, Bacterial
- Chromosome Mapping
- Base Sequence
- Bacillus subtilis
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LaFauci, G., Widom, R. L., Eisner, R. L., Jarvis, E. D., & Rudner, R. (1986). Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol, 165(1), 204–214. https://doi.org/10.1128/jb.165.1.204-214.1986
LaFauci, G., R. L. Widom, R. L. Eisner, E. D. Jarvis, and R. Rudner. “Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.” J Bacteriol 165, no. 1 (January 1986): 204–14. https://doi.org/10.1128/jb.165.1.204-214.1986.
LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol. 1986 Jan;165(1):204–14.
LaFauci, G., et al. “Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.” J Bacteriol, vol. 165, no. 1, Jan. 1986, pp. 204–14. Pubmed, doi:10.1128/jb.165.1.204-214.1986.
LaFauci G, Widom RL, Eisner RL, Jarvis ED, Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol. 1986 Jan;165(1):204–214.
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
January 1986
Volume
165
Issue
1
Start / End Page
204 / 214
Location
United States
Related Subject Headings
- Transformation, Bacterial
- RNA, Ribosomal
- Plasmids
- Operon
- Nucleic Acid Hybridization
- Microbiology
- Genes, Bacterial
- Chromosome Mapping
- Base Sequence
- Bacillus subtilis