Skip to main content

Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation.

Publication ,  Journal Article
Jarvis, ED; Cheng, S; Rudner, R
Published in: Genetics
December 1990

Studies on the region upstream to ribosomal operon rrnD of Bacillus subtilis led to the characterization of two of the four chromosomal junctions involved in the rearrangements (a translocation and an inversion) of the strains carrying the trpE26 mutation. Genetic analysis, by integrative mapping, showed linkage of rrnD to cysB and hisA (both on segment A) in the trpE26-type strains. Physical analysis showed that the region upstream to rrnD is now linked to the trpE-ilvA chromosome segment as demonstrated by analyzing restriction site-polymorphism between 168 and trpE26-type strains. Similar experiments confirmed the previous genetic data on linkage in these areas in strains carrying novel rearrangements derived from the trpE26-type strains: stable merodiploids and inversions. The nucleotide sequence of the area 5' to rrnD in both types of strains (168 and trpE26), the region downstream of the citG gene and the region carrying the trpE26 mutation (made available to us by D. Henner) provided evidence for the molecular basis of the differences in structure, allowed the identification of the break points and revealed the presence of a polypurine region upstream to rrnD as seen in other systems in B. subtilis. No extensive homology was found between pairs of junctions so far sequenced. The models proposed by C. Anagnostopoulos for the role of DNA sequences of intrachromosomal homology involved in the transfer of the trpE26 mutation and the formation of novel arrangements require therefore reevaluation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

December 1990

Volume

126

Issue

4

Start / End Page

785 / 797

Location

United States

Related Subject Headings

  • Transformation, Bacterial
  • Transduction, Genetic
  • Restriction Mapping
  • Polymorphism, Restriction Fragment Length
  • Operon
  • Mutation
  • Molecular Sequence Data
  • Genetic Linkage
  • Genes, Bacterial
  • Gene Rearrangement
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jarvis, E. D., Cheng, S., & Rudner, R. (1990). Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation. Genetics, 126(4), 785–797. https://doi.org/10.1093/genetics/126.4.785
Jarvis, E. D., S. Cheng, and R. Rudner. “Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation.Genetics 126, no. 4 (December 1990): 785–97. https://doi.org/10.1093/genetics/126.4.785.
Jarvis, E. D., et al. “Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation.Genetics, vol. 126, no. 4, Dec. 1990, pp. 785–97. Pubmed, doi:10.1093/genetics/126.4.785.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

December 1990

Volume

126

Issue

4

Start / End Page

785 / 797

Location

United States

Related Subject Headings

  • Transformation, Bacterial
  • Transduction, Genetic
  • Restriction Mapping
  • Polymorphism, Restriction Fragment Length
  • Operon
  • Mutation
  • Molecular Sequence Data
  • Genetic Linkage
  • Genes, Bacterial
  • Gene Rearrangement