Neuronal transfection in brain slices using particle-mediated gene transfer.


Journal Article

Difficulties in neuronal transfection continue to restrict the applicability of molecular approaches to neurobiology. Conventional transfection techniques have been of limited effectiveness, particularly in intact neural tissues. Viral vectors effectively transfect neurons both in vitro and in vivo but are labor intensive to construct, difficult to control, and often compromise cell viability. We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in intact brain slices. This approach is efficient, reliable, and does not require advanced molecular biological facilities for its application.

Full Text

Cited Authors

  • Lo, DC; McAllister, AK; Katz, LC

Published Date

  • December 1994

Published In

Volume / Issue

  • 13 / 6

Start / End Page

  • 1263 - 1268

PubMed ID

  • 7993619

Pubmed Central ID

  • 7993619

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/0896-6273(94)90412-x


  • eng