Reversibility of the mononucleate-to-multinucleate myogenic transition during amphibian limb regeneration.


Journal Article (Review)

Muscle differentiation involves the fusion of mononucleate myoblasts to form multinucleate syncytial myotubes. In order to assess reversibility of the mononucleate-to-multinucleate transition in urodele limb regeneration, myotube formation was induced in cultured newt limb blastemal cells. Myotubes were purified, replated at low density and injected with a cytoplasmic lineage tracer. In some cases nuclei of myotubes were labelled by incorporation of tritiated thymidine. Labelled myotubes were stable in culture for 6-8 weeks and no transfer to mononucleate cells was observed. The myotubes were implanted under the wound epidermis of a hindlimb blastema. Labelled mononucleate cells were observed 1 week after implantation and such cells could derive both the cytoplasmic lineage tracer and the nuclear marker. The number of such cells increased by 2-3 weeks after implantation. These results provide strong support for the reversibility of muscle differentiation during urodele limb regeneration, and raise questions about the mechanism of such a reversal.

Full Text

Cited Authors

  • Brockes, JP; Lo, DC

Published Date

  • January 1, 1994

Published In

Volume / Issue

  • 8 ( Pt 2) /

Start / End Page

  • 151 - 154

PubMed ID

  • 7958014

Pubmed Central ID

  • 7958014

Electronic International Standard Serial Number (EISSN)

  • 1476-5454

International Standard Serial Number (ISSN)

  • 0950-222X

Digital Object Identifier (DOI)

  • 10.1038/eye.1994.38


  • eng