Transplantation in miniature swine. II. In vitro parameters of histocompatibility in MSLA homozygous minipigs.

Journal Article

A selective breeding program based on serological techniques was used to establish three herds of miniature swine, each homozygous for a different allele at the major histocompatibility complex, termed MSLA. By selective immunization of these swine, high-titered antisera detecting the products of individual MSLA alleles were raised and used to confirm the genotype of offspring in these herds. Techniques for performing one-way mixed leukocyte cultures in these pigs were developed and demonstrated the presence of a strong mixed leukocyte culture locus (or loci) closely linked to the serologically defined MSLA locus. Cell-mediated cytotoxicity assays based on 51Cr release were developed to monitor the course of cellular immunity following allotransplantation and exhibited specificity identical to that predicted by the serological typing.

Full Text

Duke Authors

Cited Authors

  • Leight, GS; Sachs, DH; Rosenberg, SA

Published Date

  • March 1977

Published In

Volume / Issue

  • 23 / 3

Start / End Page

  • 271 - 276

PubMed ID

  • 140482

International Standard Serial Number (ISSN)

  • 0041-1337

Language

  • eng

Conference Location

  • United States