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Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.

Publication ,  Journal Article
McCarty, KS; Szabo, E; Flowers, JL; Cox, EB; Leight, GS; Miller, L; Konrath, J; Soper, JT; Budwit, DA; Creasman, WT
Published in: Cancer Res
August 1986

A monoclonal antibody to human estrogen receptor protein (H222 Sp gamma), amplified via immunoperoxidase techniques, was used in the analysis of estrogen receptor in 452 breast carcinomas, 100 endometrial carcinomas, and 15 melanomas. Immunohistochemical evaluation incorporated both intensity and distribution of staining (HSCORE). Quantitative estrogen receptor content was determined by dextran-coated charcoal analysis and sucrose density gradient analysis. In all cases H222 Sp gamma localized in the nucleus of target cells. A semiquantitative correlation existed between HSCORE and biochemical assays for breast and endometrial tissues. The sensitivities and specificities for HSCORE as compared to the biochemical assays ranged from 80 to 95% and from 74 to 94%, respectively. HSCORE correlated with tumor grade for breast and endometrial carcinoma. Immunohistochemical evaluation showed no specific staining in melanomas. The data suggest that immunohistochemical receptor localization provides information complementary to standard biochemical assays in the tissues studied.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 1986

Volume

46

Issue

8 Suppl

Start / End Page

4244s / 4248s

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Receptors, Estrogen
  • Oncology & Carcinogenesis
  • Melanoma
  • Immunoenzyme Techniques
  • Humans
  • Histocytochemistry
  • Female
  • Carcinoma
  • Breast Neoplasms
 

Citation

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McCarty, K. S., Szabo, E., Flowers, J. L., Cox, E. B., Leight, G. S., Miller, L., … Creasman, W. T. (1986). Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors. Cancer Res, 46(8 Suppl), 4244s-4248s.
McCarty, K. S., E. Szabo, J. L. Flowers, E. B. Cox, G. S. Leight, L. Miller, J. Konrath, J. T. Soper, D. A. Budwit, and W. T. Creasman. “Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.Cancer Res 46, no. 8 Suppl (August 1986): 4244s-4248s.
McCarty KS, Szabo E, Flowers JL, Cox EB, Leight GS, Miller L, et al. Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors. Cancer Res. 1986 Aug;46(8 Suppl):4244s-4248s.
McCarty, K. S., et al. “Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.Cancer Res, vol. 46, no. 8 Suppl, Aug. 1986, pp. 4244s-4248s.
McCarty KS, Szabo E, Flowers JL, Cox EB, Leight GS, Miller L, Konrath J, Soper JT, Budwit DA, Creasman WT. Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors. Cancer Res. 1986 Aug;46(8 Suppl):4244s-4248s.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 1986

Volume

46

Issue

8 Suppl

Start / End Page

4244s / 4248s

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Receptors, Estrogen
  • Oncology & Carcinogenesis
  • Melanoma
  • Immunoenzyme Techniques
  • Humans
  • Histocytochemistry
  • Female
  • Carcinoma
  • Breast Neoplasms