Inverse relationship between splenomegaly and stem cell compartment size in mice treated with nitrogen mustard.


Journal Article

Following the administration of similar doses of nitrogen mustard (4 mg/kg) to different strains of mice, wide variations in the subsequent degree of splenomegaly were observed, implying strain differences in the role of the spleen in the compensatory erythropoietic response to haematopoietic stress. This investigation was undertaken to determine whether or not these differences were related to the size of the haematopoietic stem cell compartment size in the various strains of mice. Groups of 4 different strains of mice (Swiss Webster, A/J, C57BL/6J and CS1/ASH) were injected i.v. with nitrogen mustard (4 mg/kg body weight) and autopsied at regular intervals up to 20 d post-injection. At autopsy, the wet weight of the spleen was determined. Subsequently, groups of the same 4 strains of mice were exposed to single doses of wholebody gamma-irradiation in the range of 500-900 rads. 9 d after gamma-irradiation the mice were autopsied, their spleens removed, and the number of endogenous spleen colonies determined. The greatest degree of splenomegaly was observed in the C57BL/6J mice. The Swiss Webster mice showed no splenomegaly during the time period studied. There existed a linear inverse relationship between the maximum degree of splenomegaly observed and the dose of wholebody gamma-irradiation required to completely eliminate endogenous spleen colonies. This data is in accord with the hypothesis that there exists an inverse relationship between the extent of splenomegaly observed following haematopoietic stress and the haematopoietic stem cell compartment size.

Full Text

Duke Authors

Cited Authors

  • Jensen, RH; Sharp, JG; Zajic, GH; Anderson, RW

Published Date

  • April 1, 1977

Published In

Volume / Issue

  • 18 / 4

Start / End Page

  • 309 - 316

PubMed ID

  • 857293

Pubmed Central ID

  • 857293

International Standard Serial Number (ISSN)

  • 0036-553X

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0609.1977.tb01201.x


  • eng

Conference Location

  • Denmark