Deficiency in Epstein-Barr virus receptors on B-lymphocytes of preleukemia patients.

Journal Article

Lymphocytes from eight preleukemia patients were exposed to Epstein-Barr virus (EBV) in vitro in an attempt to establish lymphoblastoid cell lines. No signs of viral infection were detected, and no cell lines were obtained. Studies using fluorescein-labeled EBV and flow cytometry revealed an unusual and consistent deficiency in EBV receptors in all patients examined. In control studies, about 15% of the unseparated lymphocytes from healthy donors bound fluorescein-labeled EBV. In spite of the lack of EBV receptors, B-lymphocytes amounted to 10 to 20% of the preleukemia lymphocyte populations, a proportion similar to that in healthy donors. When lymphocytes from preleukemic patients were first implanted with functional EBV receptors and then exposed to EBV, synthesis of EBV-determined nuclear, early, and viral capsid antigens was induced. Subsequently, several cell lines originating from preleukemic patients' lymphocytes were established. These lines are of a B-lymphocyte origin and carry EBV genome. They will provide experimental material for the molecular analysis of lymphocytic defects in preleukemia and their possible role in the transition to acute leukemia.

Full Text

Duke Authors

Cited Authors

  • Volsky, DJ; Anderson, RW

Published Date

  • August 1983

Published In

Volume / Issue

  • 43 / 8

Start / End Page

  • 3923 - 3926

PubMed ID

  • 6602654

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States